Changes of main taste substances during the growth of Gannan navel oranges
Targeted metabolomics technology was used to accurately quantify the major sugars,organic acids and amino acids during the growth of Gannan navel orange.The results revealed significant variations in growth physiology and related me-tabolite characteristics at different growth stages.The weight of single fruit gradually increased and the fruit shape index slightly decreased.As the fruit matured,sugars accumulated in large quantities,with sucrose having the highest content,and fructose and glucose having comparable contents.Most of the organic acids were gradually degraded during fruit ripening,and the total organ-ic acid content decreased,with citric acid dominating,accounting for more than 90％of the total of the six major organic acids.Aspartic acid was the main amino acid,while methionine,arginine and proline showed an upward trend,and the rest of the ami-no acids as a whole exhibited a downward trend.The sugar-acid ratio first increased and then decreased,finally reached the opti-mal ratio range,indicating that the fruit continuously improved its flavour quality during the growth process.Correlation analysis revealed that the flavour profile substances during the growth process of Gannan navel orange included fructose,glucose,quinic acid,leucine,alanine,glutamic acid and glycine.The metabolic network was constructed and the heat map analysis was conduc-ted,showing significant variation in the expression of flavor-related metabolites in the flesh of Gannan navel orange at different growth stages,reflecting the influence of the growth period on growth metabolism and flavor quality.Besides studying the met-abolic changes of the main flavour substances at different growth stages,this paper also carried out flavour evaluation and con-structed metabolic networks of related metabolites,deepening the understanding of the characteristics of the nutrient and flavour effective components of Gannan navel oranges.This research is beneficial for developing functional navel orange products and ca-pable of providing an important reference for navel orange breeding to improve the quality of the fruits.
万方数据
维普
