Danio rerio TDP43 negatively regulates the expression of type Ⅰ IFN
TDP43,a DNA/RNA binding protein belonging to the heterologous ribonucleoprotein family,plays an important role in the regulation of transcription,mRNA stability,and translation.Recent studies showed the involvement of mammalian TDP43 in inflammation and immune response,yet the mechanism governing TDP43's function in immune response remained unclear.This study delved into the role of zebrafish TDP43 in innate immune response.Firstly,amino acid alignment and phylo-genetic tree showed that TDP43 has a high similarity(over 60％)in mammals and fish.Upon infection with SVCV,the expres-sion of TDP43 in zebrafish and CIK cells exhibited an upregulation in various organs,including the brain,eyes,intestine,gills,skin,heart,liver,and kidney,as well as CIK cells.Overexpression of TDP43 in CIK cells can inhibit the expression of type Ⅰ in-terferon,while knockdown of TDP43 led to an upregulation of type Ⅰ interferon.In addition,subcellular localization analysis showed that TDP43 was mainly located in the nucleus,indicating that TDP43 might control innate immune response by regula-ting gene transcription in the nucleus.In summary,this study offered insights into the role of zebrafish TDP43 in responding to viral infections.
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