Effect of Vc on the physicochemical properties during the stable phase of freshly prepared chitosan-selenium nanoparticles
The synthesis of chitosan-selenium nanoparticles(CS-SeNPs)through in situ reduction of selenite-Vc using chi-tosan(CS)as a template were extensively investigated.However,it was important to consider the potential influence of Vc on the stable phase of freshly prepared CS-SeNPs,and not just its role in reducing selenite.In this study,we prepared CS(l)-SeNPs and CS(h)-SeNPs utilizing chitosan with varying molecular weights(3 kDa and 200 kDa)as templates,respectively.Firstly,we compared and characterized the differences in microscopic morphology and chemical structure between these two types of nano-particles,as well as to investigate changes in particle size,ζ-potential,microscopic morphology,and elemental composition of the CS-SeNPs over time following removal of Vc treatment.Furthermore,we analyzed transformation of selenium's crystalline structure and the chemical structure of CS-SeNPs during stable phase of freshly prepared CS-SeNPs by using X-ray diffraction(XRD)and Fourier Transform Infrared Spectroscopy(FTIR).The results demonstrated that when Vc was removed on the first day of stable phase,the particle sizes of both CS-SeNPs increased initially but remained stable thereafter.Additionally,ζ-poten-tial gradually decreased over time;however,after 14 days,the removement of Vc caused aggregation of CS(l)-SeNPs,while had less impact on CS(h)-SeNPs.Moreover,our findings indicated that Vc could influence both the chemical structure within the system by affecting O-H and N-H hydrogen bonding interactions among sugar chain molecules during stable phase of CS-SeNPs as well as selenium's crystalline structure.This study presents novel insights into the stable phase of freshly prepared nanoparticles from Vc or other reducing small molecules when employed in an in-situ approach.
万方数据
维普
