Purification of crude tea saponin and its effect on separation of active components related with alcohol dehydrogenase
After the purification of crude tea saponin through the method of n-butyl alcohol extraction or macroporous resin adsorption,the impact of purification on the separation of alcohol dehydrogenase related active components(AAC)from purified tea saponin by magnetic immobilization enzyme was investigated.The results demonstrated a reduction in polysaccharides and polyphenols in the purified samples,while significantly increasing the content of tea saponin.Specifically,the content of tea saponin in the purified samples obtained through extraction method and macroporous resin adsorption method was 19.8%and 18.5%higher than that of crude tea saponin,respectively.When these purified samples were utilized for the separation by magnetic immobilization,it effectively minimized enzyme activity loss ratio associated with immobilized enzymes.Notably,macroporous resin adsorption exhibited the lowest loss ratio at 27.8%.Spectral characteristics analysis revealed no changes in AAC obtained before and after purification;they remained predominantly composed of tea saponins.Furthermore,there were no significant alterations observed in AAC's inhibitory effect on alcohol dehydrogenase;this inhibitory effect remained concentration-dependent as well.Therefore,purifying crude tea saponin can reduce enzyme consumption during magnetic immobilized enzyme separation process while enhancing reusability without significantly affecting AAC.
NETL
NSTL
万方数据
