玉米秸秆纤维素降解菌的分离纯化与产酶工艺优化
向殿军 1满丽莉 1张春凤 1张彩玲 1张娣 1殷奎德 2曹天旭 1王丽娜3
作者信息
- 1. 黑龙江农业经济职业学院，黑龙江牡丹江 157041
- 2. 黑龙江八一农垦大学生命科学技术学院，黑龙江大庆 163319
- 3. 黑龙江省农业科学院大庆分院，黑龙江大庆 163319
- 折叠
摘要
从富含腐烂玉米秸秆的土壤中分离筛选到1株能降解纤维素的细菌K01，经形态观察和16S rDNA序列分析，初步鉴定该菌属于美洲爱文氏菌。对该菌产酶条件进行优化，结果表明在接种种龄24 h，培养液初始pH值7，接种量3%，培养时间48 h的优化条件下，酶活力达1.58 IU/mL。该菌株对玉米秸秆降解效果较好，优化条件下7 d降解率达56.1%。
Abstract
A cellulose-decomposing fungus strain is isolated from soil containing rich rotten corn straw. Based on morphology and 16Sr DNA sequence analysis, it is initially classified into the genus of Penicillium. And the optimum conditions for CMCase production are determined as follows: seed age of 24 hours, initial pH value of medium of 7, inoculum amout of 3%, culture time of 48 hours. Under the optimal conditions, the CMCase activity reaches 1.58 IU/mL. The strain K01 effectively degrades the corn straw, and the degradation rate reaches 56.1%in seven days.
关键词
玉米秸秆/纤维素降解菌/16S/rRNA/产酶条件Key words
corn straw/cellulose-degrading fungus/16S rRNA/enzyme-producing conditions引用本文复制引用
基金项目
牡丹江市科学技术计划项目(G2013n0012)
出版年
2014
NETL
NSTL
维普
万方数据