贵州灵芝三萜提取及其配制酒抗氧化性研究
Study on the Extraction of Triterpenoids from Ganoderma lucidum and the Antioxidant Activity of its Prepared Liquor
刘泽华 1周媛 1刘航 1胡伦 1喻仕瑞 1郑华艳1
作者信息
- 1. 茅台学院食品科学与工程系,贵州仁怀 564507
- 折叠
摘要
以贵州灵芝为原材料,采用有机溶剂浸提法提取其有效成分灵芝三萜,通过单因素试验和正交试验得到优化工艺参数,在此基础上制备灵芝三萜配制酒并考查其稳定性和抗氧化性.结果表明,浸提法最优工艺条件为提取温度75 ℃,料液比1∶35,乙醇体积分数75％,提取时间2.0 h,在此条件下灵芝三萜得率为1.15％.抗氧化试验结果表明,灵芝三萜酒对DPPH自由基、羟自由基和ABTS+自由基清除率分别为85.2％,36.8％和63.5％,Fe3+还原力为0.965,表明灵芝三萜配制酒具有较好的抗氧化能力.为品质可控的灵芝三萜配制酒的开发和应用提供了基础数据.
Abstract
Tri terpenoids,the active components of Ganoderma lucidum,were extracted from the fungus by organic solvent extraction method,and the optimal process parameters were obtained by single-factor and orthogonal experiments.Furthermore,the antioxidant activity and stability of the liquor prepared with Ganoderma lucidum tri terpenoids were also investigated.The results indicated that the optimal extraction conditions were as follows:extraction temperature of 75 ℃,solid-liquid ratio of 1∶35,ethanol concentration of 75％,and extraction time of 2.0 h.Under these conditions,the highest yield of Ganoderma lucidum triterpenoids was 1.15％.The scavenging rates of DPPH,hydroxyl radicals,ABTS+·and Fe3+reducing power of the liquor were 85.2％,36.8％,63.5％and 0.965,respectively,indicating that the liquor prepared with Ganoderma lucidum triterpenoids had good antioxidant capacity.This paper provided theoretical basis for the development and application of quality-controllable liquor prepared with Ganoderma lucidum tri terpenoids.
关键词
灵芝/灵芝三萜/浸提/配置酒/抗氧化性Key words
Ganoderma lucidum/triterpenoids/extraction/prepared liquor/antioxidation activity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据