Optimization of the Enzyme Extraction Process of Broussonetia papyrifera Leaf by Response Surface Methodology
Cellulase was investigated to extract polysaccharides from Broussonetia papyrifera leaf,the effects of solid/liquid rate,temperature,time and cellulase dosage on the yield of polysaccharides were studied.The optimal process conditions were obtained through response surface and solid/liquid rate was 1∶30(g∶mL),temperature was 58 ℃,time was 160 min and cellulase dosage was 0.95％,under this condition the polysaccharide yield was 17.25％,which verified that the test results were consistent with the predicted values of the model.
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