响应面法优化构树叶多糖酶法提取工艺的研究
Optimization of the Enzyme Extraction Process of Broussonetia papyrifera Leaf by Response Surface Methodology
张晓娜 1赵曼 1郭鹏辉1
作者信息
摘要
利用纤维素酶法提取构树叶多糖,考查了料液比、提取温度、提取时间和纤维素酶添加量4个单因素对多糖得率的影响.在此基础上,利用响应面法优化构树叶多糖的酶法提取工艺.响应面得到的最佳工艺条件为料液比1∶30(g∶mL),提取温度58 ℃,提取时间160 min,纤维素酶添加量0.95％,在此条件下,多糖提取率为17.25％,验证试验结果与模型预测值相符.
Abstract
Cellulase was investigated to extract polysaccharides from Broussonetia papyrifera leaf,the effects of solid/liquid rate,temperature,time and cellulase dosage on the yield of polysaccharides were studied.The optimal process conditions were obtained through response surface and solid/liquid rate was 1∶30(g∶mL),temperature was 58 ℃,time was 160 min and cellulase dosage was 0.95％,under this condition the polysaccharide yield was 17.25％,which verified that the test results were consistent with the predicted values of the model.
关键词
构树叶/多糖/酶解工艺/响应面/优化Key words
Broussonetia papyrifera leaf/polysaccharide/enzymatic hydrolysis process/response surface/optimize引用本文复制引用
出版年
2024
NETL
NSTL
万方数据