Screening and Validation of Hypoglycemic Active Ingredients of Yam Based on Network Pharmacology
Virtual screening and molecular docking methods were used to screen out the active ingredients of Chinese yam in the treatment of diabetes,and pharmacodynamic experiments were carried out to reveal the pharmacodynamic substance basis of Chinese yam.The 12 known chemical components in yam were constructed as molecular libraries,and virtual docking was conducted with the target models determined according to their efficacy.Then the effects of the selected multi-target active components on the animal model of alloxouracil diabetes were investigated.The results showed that:① the compounds with theoretical activity against 11β-hydroxysteroid dehydrogenase(11β-HSD1),cyclooxygenase(PTGS2)and serine protease(PRSS1)were much more than other targets;②Some components showed strong theoretical activity against multiple targets;③The results of pharmacodynamic experiments showed that Diosgenin,Stigmasterol,Brassinosteroids and arbutin,among the active ingredients identified by virtual selection,had significant effects on the animal model of alloxouracil nitrate diabetes.This project provided a new perspective and a quick way to explain the pharmacodynamic material basis of food and drug ho-mologous substances.
NETL
NSTL
万方数据
