首页|自然发酵猕猴桃果酒中产香酵母的筛选及发酵性能研究

自然发酵猕猴桃果酒中产香酵母的筛选及发酵性能研究

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以课题组前期从自然发酵猕猴桃果酒中分离的菌种为研究对象,筛选产香性能、产气性能及发酵性能较好的菌种,以期为生产高品质猕猴桃果酒提供优良菌种.结合感官评价、总酯含量测定、发酵性能评价筛选出具有优良气味且产气能力好的菌种,对菌种进行形态学观察、生理生化试验及分子生物学鉴定.采用感官评价初筛获得6株产香和产气能力较强的菌株,其大多为乳白色,呈圆形,表面干燥不透明;通过发酵性能评价,最终获得4株产香和产气能力及耐受性能较好的菌种A1、B1、JM4和TJ-2,其中A1和JM4的最佳碳源为乳糖,最佳氮源为酵母膏;B1和TJ-2的最佳碳源为麦芽糖,最佳氮源为酵母膏;鉴定4株菌均为异常威克汉姆酵母菌(Wickerhamomyces anomalu).获得4株产香和产气能力及耐受性能较好的菌种A1、B1、JM4和TJ-2,这4株菌均为异常威克汉姆酵母菌,可应用于果酒的生产中.
Screening and Fermentation Performance of Dominant Microorganism in Naturally Fermented Kiwi Wine
This study aimed to enhance the aroma quality of kiwi wine and achieve standardized production by improving aroma yield and fermentation performance.Sensory evaluation and measurement of total ester content were used to select strains.Morphology,physiology,biochemistry,and molecular biology analyses were conducted.The results showed that six strains exhibiting strong aroma production and gas production capacity were identified based on sensory evaluation.These strains appeared milky white,round,dry,and opaque on the surface.After fermentation performance evaluation,four strains with good aroma production,gas production capacity,and tolerance were selected.A1 and JM4 performed best with lactose as the carbon source and yeast paste as the nitrogen source,while B1 and TJ-2 performed best with maltose as the carbon source and yeast paste as the nitrogen source.These strains were identified as the closest genetically related strains to Wickerhamomyces anomalus.Four strains A1,B1,JM4 and TJ-2 with good aroma and gas production capacity and tolerance were obtained.These four strains are all Wickerhamomyces anomalu,which can be used in the production of fruit wine.

dominant bacteriascreening and identification of strainskiwi winefermentation performance

吴瑞章、陈兰、周清丽、周艳、周绍琴

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贵州医科大学,贵州贵阳 550025

贵州医科大学公共卫生与健康学院,贵州贵阳 550025

环境污染与疾病监控教育部重点实验室,贵州贵阳 550025

优势菌 菌株筛选与鉴定 猕猴桃果酒 发酵性能

2024

农产品加工
山西省农业机械化科学研究院 山西省农产品加工装备技术管理站 山西省农业产业化协会

农产品加工

影响因子:0.648
ISSN:1671-9646
年,卷(期):2024.(20)