Study on Extraction and Antioxidant Activity of Total Flavonoids from Different Mango Varieties
This paper focused on the optimal extraction conditions and antioxidant activity of total flavonoids in the peel and pulp of Siji mango and Daqing mango as raw materials.Selecting ultrasonic assisted extraction method to extract total flavonoids from mangoes,exploring the effects of solid-liquid ratio,ethanol concentration,ultrasonic time,ultrasonic temperature,and ultrasonic power on the yield of total flavonoids.The results indicated the optimal conditions for extracting total flavonoids in Siji mango peel were solid-liquid ratio of 1∶30(g∶mL),ethanol concentration of 70％,and ultrasound treatment for 30 minutes at a temperature of 70 ℃,ultrasound power of 252 W,and total flavonoid yield of 12.77％.Similarly,the optimal extraction process conditions for total flavonoids in Siji mango pulp were solid-liquid ratio of 1∶20(g∶mL),ethanol concentration of 30％,and ultrasound treatment for 20 minutes at a temperature of 60 ℃,ultrasound power of 252 W,and total flavonoid yield of 2.17％.The optimal extraction process conditions for total flavonoids in Daqing mango peel were solid-liquid ratio of 1∶30(g∶mL),ethanol concentration of 70％,and ultrasound treatment for 50 minutes at a temperature of 30 ℃,ultrasound power 252 W,and total flavonoid yield of 7.58％.The optimal extraction process conditions for total flavonoids in Daqing mango pulp were solid-liquid ratio of 1∶30(g∶mL),ethanol concentration of 70％,and ultrasound treatment for 30 minutes at a temperature of 40 ℃,ultrasound power 252 W,and total flavonoid yield of 1.86％.The optimal extraction process conditions obtained could effectively extract total flavonoids from mangoes.The total flavonoids in the peel and pulp of Siji mango and Daqing mango had good scavenging ability for OH and DPPH·,with clearance rates of 67.44％and 45.95％,58.57％and 42.89％,64.02％and 52.91％,68.68％and 49.07％,respectively.This paper provided evidence and support for the comprehensive development and application of mangoes and their waste,and provided reference for further research and application of natural antioxidants.
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