Heterologous Expression and Enzymatic Properties of Pectin Lyase BpPL1 from Bacillus breve
Pectin lyase is a kind of lyase that degrades pectin through the β-elimination mechanism,which is of great signifi-cance for pectin degradation and modification.In this study,a novel pectin lyase BpPL1 was heterologous expressed from Bacillus breve by genetic engineering technology,and its enzymatic properties and functions were studied further.The results showed that the protein concentration and specific activity of BpPL1 were 0.24 mg/mL and 164.61 U/mg,respectively.And the optimal pH and temperature were 9.5 and 50 ℃.In addition,5 mmol/L Ca2+could effectively increase the enzyme activity of BpPL1 to 147.2％.Using peel pectin as a substrate,BpPL1 could partially degrade it to produce oligosaccharides.The results in this study were conducive to the degradation of pectin and the preparation of oligosaccharides.
pectin lyaserecombinant expressionseparation and purificationenzymatic properties
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