Study on Ultrasonic-assisted Extraction of Corn Silk Polysaccharides and Inhibition of α-glucosidase
In this experiment,corn silk was used as raw material to extract crude polysaccharides under single factor setting for enzyme addition,ultrasonic time,enzymatic hydrolysis time,solid-liquid ratio,enzymatic hydrolysis temperature,pH,ethanol addition,ultrasonic temperature and extraction times,and orthogonal experiments were carried out by selecting enzy-matic hydrolysis time,solid-liquid ratio,enzymatic hydrolysis temperature and ultrasonic time,and the effect of crude polysaccharides of corn silk on the inhibitory activity of α-glucosidase was determined.The results showed that the amount of enzyme was 2.5％ ,the ultrasonic action time was 90 min,the enzymatic hydrolysis time was 85 min,the solid-liquid ratio was 1:30,the enzymatic hydrolysis temperature was 55 ℃,the pH was 4.5,ethanol was added 80％ ,the ultrasonic action temperature was 60 ℃,and the extraction rate was the highest in one extraction.The orthogonal experimental design was used to study the factors of enzymatic hydrolysis temperature,enzymatic hydrolysis time,solid-liquid ratio and ultrasonic treatment time,and the best results were obtained,and the optimal yield was 12.40％ .The crude polysaccharide of corn silk was the highest at 4.00 mg/mL,and the inhibitory effect on α-glucosidase reached 85.60％ .
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