农产品加工2024,Issue(23) :31-35.DOI:10.16693/j.cnki.1671-9646(X).2024.23.007

紫花地丁总黄酮提取工艺及抗氧化性研究

Optimization of Extraction Process and Antioxidant Activity of Flavonoids from Viola yedoensis

韩鹿 韩远凤 黄晓楚 谢雨轩 丁巧玲
农产品加工2024,Issue(23) :31-35.DOI:10.16693/j.cnki.1671-9646(X).2024.23.007

紫花地丁总黄酮提取工艺及抗氧化性研究

Optimization of Extraction Process and Antioxidant Activity of Flavonoids from Viola yedoensis

韩鹿 1韩远凤 1黄晓楚 1谢雨轩 1丁巧玲1
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作者信息

  • 1. 广州工商学院,广东佛山 520813
  • 折叠

摘要

采用水提法及醇提法提取紫花地丁总黄酮,并对所得提取液中黄酮含量与抗氧化性进行测定.水提法中以料液比、水浴温度、水浴时间为考查因素.结果表明,紫花地丁与水的比例为1∶10,水浴温度80℃,提取时间2.0 h为最佳提取参数,此时黄酮提取量为9.96 mg/g.醇提法中以乙醇体积分数、料液比、超声时间为考查因素.结果表明,乙醇体积分数80%,料液比为1∶15,超声时间50 min为最佳提取参数,此时黄酮提取量为21.90 mg/g.对提取物进行抗氧化活性测定,结果显示水提法中的羟自由基清除率为84.43%,醇提法中的羟自由基清除率为94.90%.

Abstract

This article used water extraction and alcohol extraction methods to extract total flavonoids from Viola yedoensis,and determined the flavonoid content and antioxidant activity in the extracted solution.In the water extraction method,the material to liquid ratio,water bath temperature,and water bath time were the factors to be considered.The experimental results showed that the ratio of Viola yedoensis to water was 1∶10,the optimal water extraction parameters were water bath temperature of 80 ℃,extraction time of 2.0 hours,and the yield of flavonoids was 9.96 mg/g.Ethanol concentration,solid-liquid ratio,and ultrasound time were considered factors in the alcohol extraction method.The experimental results showed that the optimal ethanol extraction parameters were ethanol concentration of 80% ,solid-liquid ratio of 1∶15,ultrasound for 50 minutes,and flavonoid yield of 21.90 mg/g.The antioxidant activity of the extract was determined,and the results showed that the hydroxyl radical scavenging rate in the water extraction method was 84.43% .The hydroxyl radical scavenging rate in the alcohol extraction method was 94.90% .

关键词

紫花地丁/黄酮提取/抗氧化性

Key words

Viola yedoensis/total flavonoids/antioxidant activity

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出版年

2024
农产品加工
山西省农业机械化科学研究院 山西省农产品加工装备技术管理站 山西省农业产业化协会

农产品加工

影响因子:0.648
ISSN:1671-9646
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