The purpose of this study was to investigate the effects of different concentrations of biological preservative combined with UV-C irradiation on the quality of tilapia during storage.The single-factor experiments were performed with sodium alginate(SA),chitosan(Chi),sodium carboxymethyl cellulose(CMC),melatonin and UV-C irradiation.The single factor treated tilapia were vacuum-packed and placed in a constant temperature and humidity box at 4 ℃ and 50％RH for 8 days,and the changes of tilapia were observed.The optimal single factor was selected and the optimal formula was obtained by response surface method.The preservation effect of the compound preservative combined with UV-C on tilapia were discussed and evaluated by TVB-N value,pH value,protein content,total number of bacteria,hardness and sensory evaluation.The results showed that the composite preservation obtained in 0.58％SA,0.65％Chi and 0.64％CMC can delay the spoilage of tilapia,not only TVB-N,pH,protein content,total bacteria and hardness,but also the indicator of sensory evaluation.It was better than a blank group.The Box-behnken design of the preservant formula could extend the storage period of tilapia treatment group to 14 days,while tilapia CK group pairs could only be stored until 8 days.The method of this new preservative combined with UV-C had good application value and development prospect.
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