农产品加工2025,Issue(13) :33-40.DOI:10.16693/j.cnki.1671-9646(X).2025.13.008

小米酶解饮料工艺优化及品质分析

Process Optimisation and Quality Analysis of Millet Enzymatic Beverage

李若琳 刘恩魁 冀彦忠 刘敬科 李红民
农产品加工2025,Issue(13) :33-40.DOI:10.16693/j.cnki.1671-9646(X).2025.13.008

小米酶解饮料工艺优化及品质分析

Process Optimisation and Quality Analysis of Millet Enzymatic Beverage

李若琳 1刘恩魁 2冀彦忠 2刘敬科 3李红民1
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作者信息

  • 1. 河北工程大学生命科学与食品工程学院,河北邯郸 056000
  • 2. 武安市农业农村局,河北邯郸 056300
  • 3. 河北省农林科学院生物技术与食品科学研究所,河北石家庄 050051
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摘要

采用蛋白酶解技术对小米进行处理,以水解度和DPPH自由基清除率作为评估标准,确定使用碱性蛋白酶.酶解工艺的优化采用单因素试验与响应面法进行.小米酶解饮料蛋白酶解工艺优化后参数如下:酶解pH值8.5,酶解时间3.5 h,酶解温度50 ℃,酶用量4 052 U/g.随后,分析优化后小米酶解饮料的活性物质,测得多肽、黄酮和多酚质量浓度分别为2.63,132.76,290.64 mg/L;对其抗氧化能力进行研究,发现DPPH自由基、ABTS+自由基的清除率及还原力分别为61.52%、53.72%和0.243.此外,饮料中有26种挥发性风味物质,而壬醛呈味含量最高.

Abstract

Millet was treated by protease digestion technology,and hydrolysis and DPPH radical clearance were used as the evaluation criteria for the use of alkaline protease,and the optimization of enzymatic digestion process was conducted by single factor and response surface method.The results showed that the optimized parameters of the enzyme digestion process of millet were as follows:pH value 8.5,enzyme digestion time 3.5 h,enzyme digestion temperature 50 ℃,and enzymed addition amount 4 052 U/g.Subsequently,the active substances of the optimized millet drinks were analyzed,and the contents of polypeptides,flavonoids and polyphenols were 2.63,132.76,290.64 mg/L.The antioxidant capacity was studied and found that the DPPH,ABTS+free radical clearance and reducing force were 61.52%,53.72%and 0.243.In addition,there were 26 volatile flavor substances in the beverage,with the highest flavor content.

关键词

小米/酶解饮料/活性成分/抗氧化/风味物质

Key words

millet/enzyme protein beverage/active ingredients/antioxidant/flavor substances

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出版年

2025
农产品加工
山西省农业机械化科学研究院 山西省农产品加工装备技术管理站 山西省农业产业化协会

农产品加工

影响因子:0.648
ISSN:1671-9646
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