不同提取剂对竹荪提取物抑菌效果影响
Study on the bacteriostatic effect of Dictyophora indusiata extracts with different extractants
王博阳 1张宁宁 2陈丽燕 3刘春雷 3李丹3
作者信息
- 1. 福建农林大学 食品科学学院,福建 福州 350002;宁德师范学院海洋学院,福建 宁德 352100
- 2. 福建农林大学 食品科学学院,福建 福州 350002
- 3. 宁德师范学院海洋学院,福建 宁德 352100
- 折叠
摘要
以长裙竹荪为原料,选取乙酸乙酯、正丁醇、乙醇、蒸馏水等4种提取剂,分别对竹荪的菌裙和菌柄进行提取.以抑菌圈直径和最低抑菌浓度(MIC)为评价指标,研究竹荪提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌等的抑菌效果,探究不同提取剂对竹荪提取物抑菌效果的影响,筛选出最佳提取剂,探讨竹荪不同部位抑菌效果的差异.结果表明:4种提取剂提取的竹荪提取物均具有一定的抑菌性,但水提物的抑菌性很小,而乙酸乙酯提取物对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌均具有很好的抑菌效果,抑菌圈直径最大可达(30.63±0.55)mm,MIC值最低可到12.5 mg·mL-1;竹荪菌柄提取物的抑菌性优于竹荪菌裙提取物.
Abstract
Using Dictyophora indusiata as raw material,ethyl acetate,n-butanol,ethanol and distilled water were selected to extract the skirt and stalk of Dictyophora.Taking the diameter of the bacteriostatic zone and the minimum inhibitory concentration(MIC)as the evaluation index,the bacteriostatic effect of Dictyophora extract on Escherichia coli,Staphylococcus aureus and Bacillus subtilis was studied,the effects of different extractants on the bacteriostatic effect of Dictyophora extract were studied,and the best extractant was selected.Results show that:(1)the extracts of Dictyophora have certain antibacterial activity,but the antibacterial activity of aqueous extract is very small,while ethyl acetate extract has good antibacterial effect on Escherichia coli,Staphylococcus aureus and Bacillus subtilis,and the maximum diameter of bacteriostatic zone can reach(30.63±0.55)mm,MIC and the lowest can reach 12.5 mg·mL-1.(2)the antibacterial activity of Dictyophora stalk extract is better than that of Dictyophora skirt extract.
关键词
长裙竹荪/提取剂/抑菌圈直径/最低抑菌浓度Key words
Dictyophora indusiata/extractant/diameter of inhibition zone/minimal inhibitory concentration引用本文复制引用
基金项目
宁德师范学院校级项目(2022ZX410)
宁德师范学院校级项目(2021ZX504)
出版年
2024
NETL
NSTL
万方数据