Study on the bacteriostatic effect of Dictyophora indusiata extracts with different extractants
Using Dictyophora indusiata as raw material,ethyl acetate,n-butanol,ethanol and distilled water were selected to extract the skirt and stalk of Dictyophora.Taking the diameter of the bacteriostatic zone and the minimum inhibitory concentration(MIC)as the evaluation index,the bacteriostatic effect of Dictyophora extract on Escherichia coli,Staphylococcus aureus and Bacillus subtilis was studied,the effects of different extractants on the bacteriostatic effect of Dictyophora extract were studied,and the best extractant was selected.Results show that:(1)the extracts of Dictyophora have certain antibacterial activity,but the antibacterial activity of aqueous extract is very small,while ethyl acetate extract has good antibacterial effect on Escherichia coli,Staphylococcus aureus and Bacillus subtilis,and the maximum diameter of bacteriostatic zone can reach(30.63±0.55)mm,MIC and the lowest can reach 12.5 mg·mL-1.(2)the antibacterial activity of Dictyophora stalk extract is better than that of Dictyophora skirt extract.
Dictyophora indusiataextractantdiameter of inhibition zoneminimal inhibitory concentration