一种槟榔芋疫病病原菌的分离鉴定与生物学特性
Isolation,identification and biological characteristics of a pathogenic fungus causing areca taro disease
董晓菲 1王同帅 2匡云波 1叶祖云1
作者信息
- 1. 宁德师范学院 生物科学与工程学院,福建 宁德 352100;宁德师范学院 福建省特色药用植物工程技术研究中心,福建 宁德 352100
- 2. 宁德师范学院 生物科学与工程学院,福建 宁德 352100
- 折叠
摘要
为提高槟榔芋产量和品质,探究槟榔芋疫病病原菌的生物学特性,从福鼎槟榔芋疫病发病叶片的病斑中分离病原真菌,得到可疑菌株T5-1.经Koch's法则证病、形态学和测序鉴定,初步证明其属于小孢根霉菌(Rhizopus microsporus).生物学特性研究发现:其菌落正面为灰白色,反面为黄褐色,菌丝毛绒状延展,边缘整齐,黑色孢子囊.实验结果表明,该病原菌在pH=5～9的培养基上均能生长,最适为pH=6,培养温度在4～45℃时均能生长,最适温度为40℃.
Abstract
In order to improve the yield and quality of areca taro,and to explore the biological characteristics of the pathogen of areca taro disease,pathogenic fungi were isolated from spots of the diseased leaves of Fuding areca taro,and the suspected strain T5-1 was obtained.Through Koch's rule for disease identification,mor-phology,and sequencing,it was preliminarily proved that it belongs to Rhizopus microsporus.Research on bio-logical characteristics has found that the front of its colony is grayish-white,while the back is yellowish-brown.The hyphae are fluffy and extends,with neat edges and black spore sacs.The experimental results show that the pathogen can grow in the medium of pH=5-9,with an optimal pH=6.It can also grow at 4-45℃,with an optimal temperature of 40℃.
关键词
槟榔芋/真菌/小孢根霉菌/生物学特性Key words
areca taro/fungus/Rhizopus microsporus/biological characteristics引用本文复制引用
基金项目
福建省自然科学基金项目(2022J011210)
宁德师范学院中青年科研项目(2022ZQ108)
出版年
2024
NETL
NSTL
万方数据