Objective To explore the expression of family with sequence similarity 83 member A(FAM83A)in colorectal cancer,and the effect of FAM83A knockdown on the proliferation of colorectal cancer cells and the related mechanism.Methods The expression of FAM83A in the tissues of 102 patients with colorec-tal cancer and its adjacent tissues was detected by immunohistochemistry.HCT116 cells were divided into experi-mental group and control group.The experimental group cells were transfected with FAM83A-siRNA plasmid,and the control group cells were transfected with MOCK-siRNA plasmid.The mRNA content of FAM83A in each group was detected by fluorescence quantitative PCR.The expressions of FAM83A,P13K,p-AKT and p-mTOR in each group were detected by Western blot.CCK8 assay and clonogenesis assay were used to detect cell proliferation.Results The positive rate of FAM83A in colorectal cancer patients was 88.23％(90 cases/102 cases),and the expression rate of FAM83A in paracancer tissues was 10.78％(11 cases/102 cases).The expression rate of Fam83a in colorectal cancer tissues was significantly higher than that in paracancer tissues,with statistical signifi-cance(P＜0.001).After siRNA transfection,the mRNA expression levels of FAM83A in HCT116 cells of the exper-imental group and control group were 1.23±0.20 and 0.43±0.12,respectively,and the protein expression levels of FAM83A were 1.19±0.11 and 0.23±0.08,respectively.The expression levels of P13K were 1.21±0.17 and 0.28± 0.09,the expression levels of p-AKT were 1.35±0.23 and 0.57±0.18,and the expression levels of p-mTOR were 1.48±0.20 and 1.05±0.14.The expression of P13K,p-Akt and p-mTOR was down-regulated(all P＜0.05).The ab-sorbance of HCT116 cells in the experimental group and the control group was 1.09±0.22 and 2.21±0.27,respec-tively.The cloning rate of HCT116 cells in the experimental group and the control group was 21.6％±2.4％and 62.7％±4.1％,respectively.The proliferation ability of HCT116 cells in the experimental group decreased signifi-cantly(P＜0.05).Conclusions The expression of FAM83A is significantly increased in colorectal cancer tissues,which may be related to the malignant degree of colorectal cancer.FAM83A affects the proliferation of colorectal cancer cells through the P13K/AKT/mTOR signaling pathway.
维普
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