PRR11对甲状腺癌细胞脂肪酶H、免疫调节因子及Survivin水平的影响分析
Effect of PRR11 on Lipase H,immunomodulator and Survivin levels in thyroid cancer cells
朱厚圩 1云海芹1
作者信息
- 1. 山东省烟台市烟台山医院甲状腺外科,烟台 264003
- 折叠
摘要
目的 探讨富含脯氨酸蛋白11(proline-rich protein 11,PRR11)对甲状腺癌细胞脂肪酶H(li-paseH,LIPH)、免疫调节因子及Survivin水平的影响.方法 回顾性分析2020年6月至2022年10月山东省烟台市烟台山医院甲状腺外科就诊并接受甲状腺癌手术的30例患者,男13例,女17例.根据研究方案将正常甲状腺上皮细胞作为对照组,将SW579甲状腺癌细胞分为空载组、PRR11基因上调组、PRR11基因下调组.取其癌组织及癌旁组织,使用MTT法检测各组细胞中SW579细胞增殖、酶联免疫吸附法(ELISA)检测TNF-α、IL-1β、IL-6水平,使用流式细胞术检测各组细胞中SW579细胞凋亡及CD4+CD25+、CD8+CD28-表达和使用免疫印迹分析检测Survivin蛋白表达,并进行三组比较.结果 甲状腺癌组织中PRR11mRNA表达量为1.54±0.34,显著高于癌旁组织的1.02±0.54和正常甲状腺上皮细胞系NTHY-ORI 3-1(1.02±0.65)(P＜0.05),其中 SW579 的 PRR 1 1 mRNA 表达量为 2.54±0.87,高于 K1 CELL 的 1.74±0.45 及 IHH-4 CELL细胞的1.86±0.55(P＜0.05),故选取SW579作为此次实验细胞.对照组与空载组SW579细胞增殖、凋亡、LIPH、TNF-α、IL-1β、IL-6、CD4+CD25+、CD8+CD28-、Survivin蛋白表达比较差异无统计学意义(P＞0.05),与空载组比较,PRR11基因上调组细胞增殖率则升高(P＜0.05),SW579细胞凋亡率、CD4+CD25+、CD8+CD28-占比降低(P＜0.05);PRR 11 基因下调组SW579细胞增殖率、LIPH(6.45±1.34)、TNF-α(1.85±0.36)、IL-1β(96.47±6.44)、IL-6(1.43±0.86)、Survivin蛋白表达降低且低于对照组与空载组(P＜0.05),SW579细胞凋亡率、CD4+CD25+、CD8+CD28-占比升高且高于对照组与空载组(P＜0.05).结论 下调PRR11能降低LIPH表达,调节免疫调节因子及Survivin水平,增强机体的免疫力及抑制甲状腺癌细胞的增殖、促进癌细胞的凋亡,可作为甲状腺癌诊断、治疗及预后提供新策略.
Abstract
Objective To investigate the effects of PRR11 on lipase H,immunomodulator and Survivin levels in thyroid cancer cells.Methods A retrospective analysis was performed on 30 patients(13 males and 17 females)who received surgery for thyroid cancer in the Department of Thyroid Surgery of Yantai Yantaishan Hospi-tal from Jun.2020 to Oct.2022.According to the study protocol,normal thyroid epithelial cells were used as the control group,and SW579 thyroid cancer cells were divided into no-load group,PRR11 up-regulated group and PRR11 down-regulated group.SW579 cell proliferation was detected by MTT assay and the levels of TNF-α,IL-1βand IL-6 were detected by enzym-linked immunosorbent assay(ELISA).Apoptosis of SW579 cells and expres-sions of CD4+CD25+and CD8+CD28-were detected by flow cytometry,and Survivin protein expression was detect-ed by western blot analysis.Results The mrna expression level of PRR11in thyroid carcinoma tissues(1.54±0.34)was significantly higher than that in para-carcinoma tissues(1.02±0.54)and normal thyroid epithelial cell line NTHY-ORI 3-1(1.02±0.65)(P＜0.05).The mRNA expression level of PRR11 in SW579(2.54±0.87)was higher than that in K1 CELL(1.74±0.45)and IHH-4 CELL(1.86±0.55)(P＜0.05),so SW579 was selected as the experimental cell.The expressions of SW579 cell proliferation,apoptosis,LIPH,TNF-α,IL-1β,IL-6,CD4+CD25+,CD8+CD28-and Survivin were significantly lower than those of no-load group(P＞0.05),but higher than those of no-load group(P＜0.05).SW579 cell apoptosis rate,CD4+CD25+,CD8+CD28-ratio decreased(P＜0.05).The proliferation rate,LIPH(6.45±1.34),TNF-α(1.85±0.36),IL-1β(96.47±6.44),IL-6(1.43±0.86)and Sur-vivin protein expression of SW579 cells in PRR11 down-regulated group were lower than those in control group and no-load group(P＜0.05).The apoptosis rate,CD4+CD25+and CD8±CD28-of SW579 cells were higher than those of control group and no-load group(P＜0.05).Conclusion Down-regulation of PRR11 can reduce the expression of LIPH,regulate the level of immunomodulator and Survivin,enhance the body's immunity,inhibit the prolifera-tion and promote the apoptosis of thyroid cancer cells,and provide a new strategy for the diagnosis,treatment and prognosis of thyroid cancer.
关键词
富含脯氨酸蛋白11(PRR11)/甲状腺癌/脂肪酶H/免疫调节因子/SurvivinKey words
Proline-rich protein 11(PRR11)/Thyroid cancer/Lipase H/Immunomodulator/Survivin引用本文复制引用
出版年
2024
NETL
NSTL
万方数据