白细胞介素-6/信号转导和转录激活因子-3信号通路关键分子与乳腺癌易感性关系
Relationship between key molecules of IL-6/STAT3 signaling pathway and breast cancer susceptibility
陈为军 1王晓丹 1都婧 2李丽 1巩立国3
作者信息
- 1. 烟台市烟台山医院肿瘤内科,烟台 264000
- 2. 烟台市烟台山医院检验科,烟台 264000
- 3. 烟台市烟台山医院乳腺外科,烟台 264000
- 折叠
摘要
目的 探讨白细胞介素-6/信号转导和转录激活因子-3(interleukin-6/signal transducer and acti-vator of transcription 3,IL-6/STAT3)信号通路关键分子与乳腺癌易感性关系.方法 采用病例对照研究设计,选取2021年3月至2023年3月烟台市烟台山医院乳腺外科收治的136例乳腺癌患者作为观察组,按照1:1比例匹配同年龄的136例健康体检者作为对照组.比较2组临床资料、IL-6/STAT3信号通路关键分子,采用条件Logistic回归及相加作用分析评估乳腺癌易感的风险因素,绘制受试者工作特征曲线(receiver operat-ing characteristic curve,ROC)及曲线下面积(area under curve,AUC)分析诊断效能.结果 观察组与对照组体质指数(body mass index,BMI)(22.21±0.68 vs.21.30±0.70)、月经初潮年龄(13.50±1.24 vs.14.83±1.42)、IL-6(3.50±1.05 vs.2.41±0.74)、蛋白酪氨酸激酶 1(janus protein kinase 1,JAK1)mRNA(1.23±0.36vs.0.88±0.26)、STAT3 mRNA(1.68±0.50 vs.1.17±0.35)、绝经(30.15％vs.55.88％)、母乳喂养(82.35％vs.92.65％)及体育锻炼(44.12％vs.58.09％)等比较,差异均有统计学意义(t=10.87、8.23、9.89、9.19、9.75,x2=18.37、6.59、5.31,P＜0.05).母乳喂养[比值比(odd ratio,OR):0.550]、IL-6(OR:4.409)、JAK1(OR:5.370)及STAT3(OR:4.386)是乳腺癌易感风险因素(P＜0.05).ROC曲线显示,IL-6、JAK1、STAT3mRNA联合诊断乳腺癌效能明显优于单一诊断效能;IL-6、母乳喂养同时暴露者乳腺癌发病率是非暴露者的3.508倍;JAK1、母乳喂养同时暴露者乳腺癌发病率是非暴露者的4.136倍;STAT3、母乳喂养同时暴露者乳腺癌发病率是非暴露者的3.537倍.结论 IL-6/STAT3信号通路关键分子、母乳喂养均是乳腺癌发病易感因素,两者存在相加交互作用,从而增加乳腺癌易感性,对该病鉴别诊治有显著意义.
Abstract
Objective To investigate the relationship between key molecules of interleukin-6/signal trans-ducer and activator of transcription 3(IL-6/STAT3)signaling pathway and breast cancer susceptibility.Methods A case-control study design was adopted.136 patients with breast cancer admitted to Department of Breast Surgery,Yantai Yantaishan Hospital from Mar.2021 to Mar.2023 were selected as the case group,and 136 healthy subjects of the same age were matched as the control group in a 1:1 ratio.The clinical data and key molecules of IL-6/STAT3 signaling pathway were compared between the two groups.The risk factors of breast cancer susceptibility were evaluated by conditional Logistic regression and addition analysis,and the diagnostic efficiency of receiver op-erating characteristic curve(ROC)and area under curve(AUC)were plotted.Results The body mass index(BMI)(22.21±0.68 vs.21.30±0.70 kg/m2),age at menarche(13.50±1.24 years vs.14.83±1.42 years),IL-6(3.50±1.05 vs.2.41±0.74),Janus protein kinase 1(JAK1)mRNA(1.23±0.36 vs.0.88±0.26),STAT3 mRNA(1.68±0.50 vs.1.17±0.35),and menopause(30.15％vs.55.88％),breastfeeding(82.35％vs.92.65％),physical exercise(44.12％vs.58.09％)were significantly different between the case group and the control group(t=10.87,8.23,9.89,9.19,9.75,x2=18.37,6.59,5.31,P＜0.05).Breastfeeding[odd ratio(OR):0.550],interleukin-6(IL-6)(OR:4.409),janus protein kinase 1(JAK1)(OR:5.370)and signal transducer and activator of transcription 3(STAT3)(OR:4.386)mRNAs were risk factors for breast cancer susceptibility(P＜0.05).ROC curve showed that the combined diagnostic efficacy of IL-6,JAK1,STAT3 mRNA in breast cancer was significantly better than that of a single diagnostic efficacy.The incidence rate of breast cancer in those who were exposed to IL-6 mRNA and breast feeding was 3.508 times higher than that in those who were not exposed.The incidence rate of breast cancer in those who were exposed to JAK1 mRNA and breast feeding at the same time was 4.136 times higher than that in those who were not exposed.The incidence rate of breast cancer in those who were exposed to STAT3 mRNA and breast feeding was 3.537 times higher than that in those who were not exposed.Conclusion The key molecules of IL-6/STAT3 signaling pathway and breast-feeding are predisposing factors of breast cancer,and they have additive interaction,thus increasing the susceptibility of breast cancer,which has significant practical significance for the differential diagnosis and treatment of this disease.
关键词
乳腺癌/病例对照研究/IL-6/STAT3信号通路Key words
Breast cancer/Case-control study/IL-6/STAT3 signaling pathway引用本文复制引用
基金项目
山东省医药卫生科技发展计划项目(202005031134)
出版年
2024
NETL
NSTL
万方数据