摘要
目的 探究ADAR1和AZIN1在胃癌患者癌组织与癌旁组织中的表达及其临床意义研究.方法 选取2020年1月至2021年1月在济宁医学院附属医院消化内科和肿瘤科治疗的116例Ⅰ～Ⅲ期胃癌患者为研究对象.所有患者均经手术切除治疗,并经组织病理学分析确诊.分析患者胃癌组织中ADAR1、AZIN1表达与临床病理特征的关系.通过qPCR分析胃癌及癌旁组织中ADAR1和AZIN1 mRNA表达.通过免疫组化分析胃癌及癌旁组织中ADAR1和AZIN1表达.分析ADAR1和AZIN1与患者术后3年生存率的关系.通过多变量Logistic回归分析影响胃癌患者预后不良的影响因素.使用ROC曲线分析ADAR1和AZIN1表达水平在胃癌患者预后中的诊断效能.结果 ADAR1和AZIN1在未分化胃癌组织、有淋巴结转移和较高TNM分期的患者中表达水平较高,表明这两个基因的高表达与胃癌的恶性程度密切相关.qPCR分析显示,胃癌组织和癌旁组织中ADAR1 mRNA表达分别为1.96±0.18、1.12±0.03,胃癌组织和癌旁组织中AZIN1 mRNA表达分别为1.85±0.16、1.04±0.02,胃癌组织较癌旁组织升高(P＜0.05).ADAR1和AZIN1阳性患者与更低的3年生存率相关(P＜0.05).预后不良多因素Logistic回归分析中发现,胃癌低分化程度、浸润程度加深、淋巴结转移、较高的TNM分期、ADAR1阳性表达及AZIN1阳性表达均显著增加了胃癌不良预后的风险(P＜0.05).联合检测的敏感性和AUC值都高于ADAR 1、AZIN 1单独检测(P＜0.05).结论 ADAR1和AZIN1在胃癌组织中的表达显著高于癌旁组织,特别是在未分化的肿瘤、有淋巴结转移和高TNM分期的患者中.这两个基因的高表达与胃癌患者的不良预后密切相关,联合检测显示出更高的诊断效能.ADAR1和AZIN1作为胃癌发展和预后的关键生物标志物,为胃癌治疗提供了新的靶点.
Abstract
Objective To explore the expression of ADAR1 and AZIN1 in cancer tissues and adjacent tis-sues of gastric cancer patients and study their clinical significance.Methods 116 patients with stage Ⅰ-Ⅲ gastric cancer treated in the Department of Gastroenterology and Oncology from Jan.2020 to Jan.2021 were recruited as research subjects.All patients were treated with surgical resection and confirmed by histopathological analysis.All patients signed the protocol informed consent upon inclusion in the study.The relationship between ADAR1 and AZIN1 expression and clinicopathological characteristics in gastric cancer tissues of patients was analyzed.The ex-pression of ADAR1 and AZIN1 mRNA in gastric cancer and adjacent tissues was analyzed by qPCR.The expres-sion of ADAR1 and AZIN1 in gastric cancer and adjacent tissues was analyzed by immunohistochemistry.The rela-tionship between ADAR1,AZIN1 and patient survival rate 3 years after surgery was analyzed.Multivariable Logis-tic regression was used to analyze factors affecting the poor prognosis of gastric cancer patients.ROC curve was used to analyze the diagnostic performance of ADAR 1 and AZIN 1 expression levels in the prognosis of gastric can-cer patients.Results ADAR1 and AZIN1 had higher expression levels in undifferentiated gastric cancer tissue,patients with lymph node metastasis,and higher TNM staging,indicating that the high expression of these two genes was closely related to the malignancy of gastric cancer.QPCR analysis showed that the expression of ADAR1 mRNA in gastric cancer tissue and adjacent tissue was 1.96±0.18 and 1.12±0.03,respectively.The expression of AZIN1 mRNA in gastric cancer tissue and adjacent tissue was 1.85±0.16 and 1.04±0.02,respectively.The ex-pression of ADAR1 mRNA in gastric cancer tissue was higher than that in adjacent tissue(P＜0.05).ADAR1 and AZIN1 positive patients were associated with lower 3-year survival rates(P＜0.05).In the multivariate logistic re-gression analysis of poor prognosis in gastric cancer patients,low differentiation,deepening of infiltration,lymph node metastasis,higher TNM staging,positive expression of ADAR1 and AZIN1 all significantly increased the risk of poor prognosis in gastric cancer(P＜0.05).The sensitivity and AUC value of joint detection were higher than those of ADAR1 and AZIN1 alone detection(P＜0.05).Conclusions The expression of ADAR1 and AZIN1 in gastric cancer tissues is significantly higher than that in para-cancerous tissues,especially in patients with undiffer-entiated tumors,lymph node metastasis and high TNM stage.The high expression of these two genes is closely relat-ed to the poor prognosis of gastric cancer patients,and the combined detection shows higher diagnostic efficiency.Therefore,ADAR1 and AZIN1,as key biomarkers for the development and prognosis of gastric cancer,provide new targets for gastric cancer treatment.
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