Effects of different soaking and germination accelerating treatments on seed germination of Angelica keiskei
Angelica keiskei is an edible and medicinal vegetable with high economic value and broad development prospects.However,due to the limitation of soil properties,temperature and humidity conditions,the germination rate of Angelica keiskei seeds is generally low,which restricts the cultivation and production of Angelica keiskei.In this study,the seeds of Angelica keiskei,which was newly harvested in Shandong Province,were used as materials to study how to improve the germination of seeds by soaking seeds at various temperature water and exogenous addition of different concentrations of KNO3,plant hormones and strong oxidants.The results show that soaking seeds in warm water(55 ℃),KNO3 solution,plant hormones and strong oxidant treatment could promote seed germination to varying degrees.Compared with water soaking,in the KNO3 solution treatment group,the germination rate and germination potential ability of 750 mg·L-1treatment were 83.82％and 59.56％(p＜0.05),respectively,which were the best in the group.In the plant hormone treatment group,the germination rate of 100 mg·L-1 GA3 solution treatment was the highest,reaching 81.19％(p＜0.05).In the strong oxidant treatment group,the germination rate and germination potential of 500 mg·L-1KMnO4 solution were 84.92％and 46.03％,respectively(p＜0.05),which was the highest in the group.The hypocotyl length of seedlings treated with 750 mg·L-1 KNO3 solution was 1.08 cm,the root length was 2.09 cm,the hypocotyl length of seedlings treated with 100 mg·L-1 GA3 solution was 2.41 cm,the root length was 2.01 cm,and the hypocotyl length of seedlings treated with 500 mg·L-1 KMnO4 solution was 1.21 cm,the root length was 1.63 cm.Comprehensive comparison above results,we suggested that the treatment of 100 mg·L-1 GA3 solution was the best treatment due to high seed germination rate,the largest root length and hypocotyl length of the seedlings.The results of this study can provide reference for Angelica keiskei planting and breeding of similar seeds.
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