为开发碧桃转录组微卫星信息，利用454高通量测序技术，对其花瓣转录组序列进行SSR位点发掘，结果发现含SSR的序列4705条，共得到5668个SSR，平均每3．49 kb出现1个SSR。微卫星序列主要以三碱基重复为主，约占总数的42．66％。笔者共发现516种碱基重复基元，所占比例最高的为（AG／CT）n（18?34％），其次是（ AAG／CTT） n（12?42％）。微卫星多为重复长度小于20 bp 的短序列，长度大于20 bp 的微卫星仅占总数的12?13％。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关（ P＜0?05），相关系数为－0?246。
Deep sequenced-based transcriptome analysis of microsatellites in peach (Prunus persica cv. duplex) flowers
To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high?throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average densi?ty of one SSR per 3.49 kb. Tri?nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs, ( AG/CT ) n was the most frequent repeat motif ( 18?34%) , followed by the ( AAG/CTT ) n ( 12?42%) . The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation ( P<0.05) between the frequency of microsatellites and the length, the correlation coefficient was -0.246.
国家科技期刊平台
NSTL
万方数据
维普
