Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids
Activated carbon is often used to improve cell growth and development in somatic embryogenesis. In this stud?y, three types of activated carbon, including phosphoric acid activated carbon, activated carbon for refinement of injec?tion and activated carbon purchased from Sigma were added respectively to the mediums for improving somatic embryo?genesis frequency of two genotypes of cell lines in Liriodendron hybrids. The number of total somatic embryos and cotyle?don embryos were counted, pH and osmotic pressure of the mediums were detected, and the adsorption ability of activa?ted carbon were analyzed to select the most suitable activated carbonl and to find their potential effects on somatic embry?ogenesis of Liriodendron hybrids. The results showed that the first two types of activated carbon induced more total somat?ic embryos and cotyledon embryos than the third type, especially the activated carbon for refinement of injection. The ad?sorption ability of activated carbon for refinement of injection was moderate among the three, and the pH and osmotic pressure of the mediums containing it were also temperate compared with the other two types of activated carbon. It was concluded from the results that the adsorption ability of activated carbon may change the pH and osmotic pressure of me?diums directly to influence the somatic embryogenesis of Liriodendron hybrids.
国家科技期刊平台
NSTL
万方数据
维普
