The factors influencing the embryogenic callus initiation and somatic embryo yield in Pinus elliottii resistant to pine needle brown spot disease
[Objective]The somatic embryogenesis induction system of resistant Pinus elliottii is established and optimized to provide technical support for the large-scale rapid propagation of high-resistant materials of P.elliottii.[Method]The immature zygotic embryos from four families of P.elliottii are used to explore the effects of different families on the embryogenic callus initiation rate.The embryogenic callus with good proliferation rate and generating large quantity of embryo suspensor mass is selected to test somatic embryo maturation.The maturation conditions of somatic embryos are optimized based on genotype,phytosulfokine(PSK)concentration,and subculture times.[Result]A significant difference is observed in the initiation frequencies of embryogenic callus among the four families(P＜0.05).Family No.30 has the highest initiation rate(29.8±5.6)％,while family No.32 has the lowest(7.3±3.4)％.A total of 37 embryogenic cell lines with stable proliferation are obtained.Of these,25.7％of 35 cell lines produce matured somatic embryos,and the yields(number of somatic embryos)in 2007-3 and 2007-5 are significantly higher than that of other cell lines,with an average yield of(91.3±7.0)and(89.8±8.4)cotyledonary somatic embryos per mL,respectively,while the remaining 74.3％does not produce any somatic embryos.The addition of PSK significantly affects the yield of somatic embryos of resistant P.elliottii,and embryo production is considerably increased when the PSK concentration is 1 mg/L.The number of mature somatic embryos decreases with the increase in subcultures.The number of mature somatic embryos of cell lines 2007-3 gradually decreases after eight subcultures but maintains embryogenic competence in the 20th subculture.For cell lines 2007-5,the number of mature somatic embryos reduces as subcultures rise,ultimately losing maturation capacity after 20 subcultures.[Conclusion]The initiation of embryogenic callus is greatly influenced by the genotype.Genotype and PSK concentration significantly affect the somatic embryo yield of resistant P.elliottii.The yield of somatic embryos decreases with the increase in subcultures.The somatic embryogenesis technology system of resistant P.elliottii is optimized to provide technical support for the somatic embryogenesis of excellent genotypes of P.elliottii.
Pinus elliottiisomatic embryogenesisgenotypephytosulfokinesomatic embryo maturationbreeding for disease resistance
国家科技期刊平台
NSTL
万方数据
