Influence of carbon and nitrogen sources on phosphate solubilization by K3 strain
The ability of solubilization of tri-calcium phosphate(TCP)by K3 strain was studied in NBRIP medium with different carbon sources (soluble starch, sucrose, glucose, etc, ), nitrogen forms ( NO3 , NH4 +, urea) and different C/N (40:1,20-1,8:1). The results showed that K3 had the highest capacity of dissolving TCP when glucose was used as carbon source. The released soluble P from TCP was 653.00 μg·mL-1. However,the released P was only 4. 83 μg·mL-1 when the soluble starch was provided as carbon source. In addition,the released P by K3 was only 14.3 μg·mL-1 ,when nitrate was provided as nitrogen source. But it reached 460.0 μg·mL-1 when urea was provided. In contrast, ammonium significantly promoted the ability of phosphate solubilization of K3. The amount of released P was 37 fold under ammonium source in comparison with nitrate source. The P solubilization activity was closely related to the C/N. At lower C/N,K3 strain showed higher activity of P. The highest solubilization acitivity of P was attained at the C/N of 8 :1. Results also showed that the addition of soluble P in the medium repressed the ability of K3 to dissolve TCP.
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