Protective effect of green tea polyphenols on oxidative stress-induced bovine mammary epithelial cells injury
To investigate the protective effect of different concentration of green tea polyphenols ( GTP) on oxidative stress-induced bovine mammary epithelial cells injury,a model system of H2O2-induced damage to mammary epithelial cells cultured was established. Cell viability was detected by MTT assay. The content of malondialdehyde (MDA), the activities of superoxide dismutase(SOD) and lactate dehydrogenase(LDH)in the cell culture media were determined by a colorimetric method. Apoptotic cells were detected by flow cytometry. Meanwhile, the Caspase-3 relative activity was detected by spectrophotometric method. The results showed that compared with the H2O2 group,the GTP-treated groups(20-100 μg·mL-1) exhibited the increase of the cell viabilities of mammary epithelial cells and SOD activity,and decreased the levels of MDA,LDH,Caspase-3 activity and cell apoptosis. And the treatment of GTP( 100 μg·mL-1) had the most significant effect. These results suggested that a certain concentration of GTP might alleviate the injury of mammary epithelial cells induced by oxidative stress, increased the cell viability, and inhibited the cell apoptosis. Conclusion: GTP protected mammary epithelial cells against oxidative stress-induced injury,which was probably related to the attenuation of MDA content,the in-creasement of SOD activity and the inhibition of the Caspase-3 activity.
green tea polyphenolsbovineoxidative stressmammary epithelial cellsapoptosis
国家科技期刊平台
NSTL
万方数据
维普
