Proteomic analysis on root growth and development of watermelon seedlings
[Objectives] To understand the function of differentially expressed proteins in roots of watermelon seedlings, proteins of watermelon roots at different developmental stages were studied using proteomics technique.This work will provide a theoretical basis for further studying on the molecular mechanism of watermelon root development.[Methods] A comparative study of watermelon roots at different developmental stages was carried out by using two-dimensional gel electrophoresis.The differentially expressed protein spots identified by electrophoresis were further analyzed by MALDI-TOF/TOF.Expression profiles of candidate protein genes were investigated using quantitative real time PCR(RT-qPCR).[Results] Twelve differentially expressed protein spots were identified by electrophoresis.Levels of 12 proteins were differential, of which,4 proteins were up-regulated,2 proteins were down-regulated and 6 proteins were differentially-regulated.11 protein spots were finally identified by MALDI-TOF/TOF.Functions of these 11 proteins were analyzed which included 10 functional proteins involved in protein biosynthesis (20％), metabolism (40％), plant defense (30％) and GA signaling(10％), and 1 unknown protein.Four candidate protein genes were further investigated using RT-qPCR.Expression changes of jasmonate-induced protein 1 gene and ascorbate peroxidase gene in mRNA level were consistent with those in proteomic levels, while the expression of gibberellin receptor gene GID1L2 and Jasmonate-induced protein 2 gene might be related with transcriptional regulation.[Conclusions] This work gives a global insight into the characteristics of proteome of watermelon roots at different developmental stages by using two-dimensional gel electrophoresis.Proteins with important function were acquired which might be valuable resources for future research.
国家科技期刊平台
NSTL
万方数据
维普
