[Objectives]The aim of this experiment was to study the isolation and identification,resistance to common antimicrobial drugs and antibacterial activity of a strain of Bacillus subtilis of yak origin(named:TS1),and to explore the feasibility of its application as a probiotic additive in animal husbandry.[Methods]The fresh manure of healthy cattle was treated with water bath at 100℃for 10 min to screen the resistant Bacillus,and then the morphological,physiological and biochemical characteristics and 16S rRNA sequence analysis were integrated to identify them,and their biological characteristics were studied by measuring growth curves,acid and bile salt resistance tests,drug sensitivity tests and inhibition tests,and their possible production of the types of antibacterial substances they may produce.[Results]TS1 was Bacillus pumilus with acid-resistant and bile salt-resistant growth characteristics;TS1 was sensitive to 12 common antibacterial drugs and had inhibitory effects on Escherichia coli,Salmonella,Staphylococcus aureus and Streptococcus suis.Sequencing analysis showed that this strain could encode a novel antimicrobial peptide with 48.90%homology to Amylocyclicin,and thus exerted its antimicrobial activity.[Conclusions]This Bacillus pumilus TS1 has certain resistance and potential probiotic ability and broad-spectrum antibacterial effect,and has low risk of antibiotic resistance transmission in the livestock and poultry intestinal microbial system,and is suitable as a probiotic additive class antibiotic replacement product.
Bacillus pumilusisolation and identificationbacterial inhibitionalternative to antibioticsprobiotics additive
国家科技期刊平台
NSTL
维普
万方数据
