[Objectives]The purpose of this experiment was to investigate the effect of dietary curcumin supplementation on the damage of liver antioxidant properties in laying hens caused by heat stress.[Methods]One hundred and eighty 280-day-old Hylan laying hens provided by the Nanjing Panchu Mechanized Chicken Farm were taken as the research objects,and they were randomly divided into 3 groups.The control group(Con)was fed a basal diet and the ambient temperature was(24±2)℃;the heat stress group(HS)was based on the control group and maintained heat stress for more than 6 hours a day,the ambient temperature was(34±2)℃;curcumin group(HS+CUR)was based on the heat stress group and was fed basal diet supplemented with 150 mg·kg-1 curcumin;4 repetitions per group,15 chickens per replicate.In order to explore the protective effect of curcumin on the liver of heat-stressed laying hens,TMT(tandem mass tags)proteomics technology was used to analyze the differential expression of liver proteins in laying hens.[Results]The results of real-time fluorescence quantitative PCR showed that the expressions levels of SOD1 and GPX1 in the liver of the heat stress group were significantly lower than those of the control group(P<0.01),and the expression level of CAT significantly reduced(P<0.05);after adding curcumin,the expression levels of SOD1 and GPX1 in the liver significantly increased(P<0.01),and the expression level of CAT significantly increased(P<0.05).A total of 5 039 proteins were detected by TMT proteomic analysis,of which 4 235 had quantitative information.The results of TMT showed that the differentially expressed proteins were mainly involved in lipid and glucose metabolism,oxidative stress,mitochondrial dysfunction,and inflammation.The KEGG enrichment analysis showed that the PPAR signaling pathway,peroxidase Body,lipid metabolism,cytochrome P450,biosynthesis and adipocytokine signaling pathways were significantly enriched among groups.Fifteen differentially expressed proteins were selected for gene level verification,and the results showed that the gene level was consistent with the proteomics results.[Conclusions]Curcumin supplementation could alleviate heat stress-induced hepatic oxidative stress and biological processes related to lipid metabolism in laying hens.
国家科技期刊平台
NSTL
维普
万方数据
