[Objectives]This study aimed to investigate the effect of adding broad bean skin(BBS)to the diet on the barrier-related gene expression,digestive enzyme activity and microflora of the jejunum in Hu sheep.[Methods]Thirty 4-month-old male Hu sheep with similar body weight[(27±2.0)kg]were divided into two groups,feeding with basal diet(CON)and diet with 30%broad bean skin(BBS),respectively.The pre-feeding period was 10 days,and the formal period was 50 days.At the end of the experiment,jejunal tissue and chyme were collected(n=5),mucosal barrier-related gene expressions were detected by RT-qPCR,mucosal digestive enzyme activities were detected by biochemical kits,and chyme microbial was analyzed by high-throughput sequencing.[Results]Compared with CON group,the jejunal mucosal cell junction genes Claudin-1,Occludin,MUC-2 and ZO-1,pro-inflammatory factors IL-6,IL-10 and TNF-α mRNA levels in the BBS group had no significant changes(P>0.05),while the expression level of IL-1β mRNA significantly decreased(P<0.05).There was no significant change in the mucosal α-amylase,trypsin and lipase activities between two groups(P>0.05).The microbial Shannon index,Chao1 index and Observed otus index in the BBS group significantly decreased(P<0.05).At the phylum level,the relative abundance of Proteobacteria in the BBS group significantly increased(P<0.05),while the relative abundance of Firmicutes,Patescibacteria and F/B value significantly decreased(P<0.05).At the genus level,the relative abundance of Christensenellaceae R-7 group,Firmicutes unclassified,Candidatus Saccharimonas,Saccharofermentans,Lachnospiraceae NK3A20 group and Acetitomaculum significantly decreased(P<0.05).The functions of jejunal microbial differential flora were mainly enriched in the tricarboxylic acid cycle,fatty acid β-oxidation,protocatechuic acid degradation and L-leucine degradation pathways.[Conclusions]The addition of 30%BBS in the diet can decrease the jejunum mucosal inflammatory factor IL-1β mRNA expression level,change the jejunum chyme microbial flora diversity and relative abundance,and regulate intestinal fermentation environment.The results will provide a reference for the application of broad bean skin in the development of sheep industry.
国家科技期刊平台
NSTL
维普
万方数据
