[Objectives]The study aimed to investigate the neurotrophic factor tyrosine kinase B receptor(Trkb)gene effects on the pituitary gonadotropin secretion of Hu sheep.[Methods]Tissue expression profiling of Trkb was performed using qPCR.The overexpression vector of Trkb was constructed and transfected into Hu sheep pituitary cells.The effects of overexpression of Trkb on pituitary cell proliferation and gonadotropin secretion were detected by qPCR,Western blot,EdU and ELISA.[Results]Trkb was expressed in the heart,liver,spleen,lung,kidney,hypothalamus,pituitary and other tissues of Hu sheep,but the expression level in the pituitary was significantly higher than that in other tissues(P<0.05).Trkb was differentially expressed in pituitary tissues of Hu sheep at different developmental stages,and was highly expressed in pituitary tissues at 6 months of age(P<0.05),and the expression level was lower at 5 days and 3 months of age.Compared with the control group,overexpression of Trkb gene significantly promoted pituitary cell proliferation rate(P<0.05),and the expression levels of proliferation marker gene Pcna and Bcl2/Bax ratio significantly increased(P<0.05).In addition,overexpression of Trkb significantly increased the expression levels of gonadotropin-related genes Fshβ and Lhβ,and significantly promoted the secretion levels of follicle stimulating hormone(FSH)in pituitary cells(P<0.05).[Conclusions]Overexpression of Trkb could significantly promote cell proliferation efficiency of pituitary cells,decrease the level of cell apoptosis,and thus significantly increase the secretion of gonadotropin in Hu sheep.This study preliminarily verified the function of Trkb in Hu sheep pituitary cells and provided a experimental basis for further study of the molecular mechanism of Trkb regulating pituitary function.
Hu sheeppituitarytyrosine kinase B receptor(Trkb)cell proliferationgonadotropin
国家科技期刊平台
NSTL
维普
万方数据
