In vitro Culture and Rapid Propagation of Stem Segments with Buds of Lagerstroemia indica
The semi-lignified stem segments with buds of Lagerstroemia indica'Caixia Mantian'with dark pink petals were used as explants to optimize the in vitro culture system from the aspects of dis-infection and sterilization,primary medium and proliferation medium.The results showed that the ap-propriate disinfection method for stem segments of L.indica was to first soak them in 75％alcohol for 18 s,and then soak them in 5％NaClO solution for 8 min.The optimal initiation medium for stem segment with axillary buds was 1/2MS+6-BA 1.0 mg·L-1+NAA 0.1 mg·L-1+sucrose 20 g·L-1+agar 6.3 g·L-1,with an average induction rate reaching 45％,and then the buds had the best growth and the leaves was tender green in color.The L9(33)orthogonal experiment design was carried out with three factors:basic medium,6-BA concentration and KT concentration.The main and secondary factors affecting the effective proliferation coeffi-cient of L.indica tissue culture seedlings were found to be:6-BA＞basic medium＞KT.The culture media with the best effect on the proliferation and growth of the L.indica seedlings were MS+NAA 0.05 mg·L-1+6-BA 2.0 mg·L-1+KT 4.0 mg·L-1+sucrose 30 g·L-1+agar 6.3 g·L-1 and WPM+NAA 0.05 mg·L-1+6-BA 2.0 mg·L-1+KT 4.0 mg·L-1+sucrose 30 g·L-1+agar 6.3 g·L-1.
万方数据
维普
