The Effect of Antioxidant Activity on the Polysaccharides from Pleurotus cornucopiae with Different Extraction Ways
Seven different extaction methods of the polysaccharides from pleurotus cornucopiae,including traditional hot water extraction,ultrasonic extraction,ultrasonic wave complex hot water extraction,acid extraction,alkali extraction,ultrasonic wave complex acid extraction and ultrasonic wave complex alkali extraction.And antioxidant activity of the polysaccharides in virto was investigated by using reducing power,hydroxyl radical scavenge activity,superoxide radical assay and DPPH· scavenge activity,using Vc as positive control.The results showed that the polysaccharides prepared by acid extraction had the highest extraction yield that reached 6.58％.In general,the polysaccharides by ultrasonic wave complex hot water extraction reached the highest reducing power(2.18 at 10 mg·mL-1),hydroxyl radical scavenging ability was(95.56％ at 10 mg·mL-1),Superoxide anion radical scavenging activity was(62.35％ at 1 mg·mL-1),DPPH· scavenging ability was (80.32％ at 2 mg· mL-1).The antioxidant activity of the polysaccharides prepared by ultrasonic wave complex alkali extraction was also strong,but the polysaccharides prepared by ultrasonic wave complex acid extraction was the worst.It is concluded that ultrasonic-assisted hot water extraction is an effective method for extracting polysaccharides from pleurotus cornucopiae,and it's more conducive to study the biological activity of polysaccharides.
polysaccharides from Pleurotus cornucopiaeextraction yieldantioxidant activityfree radicals
国家科技期刊平台
NSTL
万方数据
维普
