With the development of high-throughput sequencing technology,more and more unculturable species of soil microorganisms have been discovered and identified.However,high-throughput sequencing cannot isolate and purify a single strain with key functions from massive databases,which has certain limitations on the mechanical research and application of key function microorganisms.In this study,soil samples of rice-crayfish cocropping and rice-single cropping were taken as the research object.The differences were compared in soil bacterial classification,microbial diversity,composition specificity,and microbial functional characteristics of traditional spread plate method,96-well culture plate in situ scribing separation,and the combination of high-throughput and cultivable methods—the"96-well high-throughput cultivable method".The results showed that comparing with the traditional spread plate method,the number of bacteria obtained by 96-well high-throughput culture method was increased by about 5 times,the identification of genus level was increased by about 4 times,and the identification of function was increased by about 2 times.It can be seen that the 96-well high-throughput culturable method greatly improves the"resolution"of the separation and identification of soil microorganisms.The results of this study made up for the shortage of strains that could not be obtained by traditional spread plate method,expanded the application field of high-throughput culturable technology,and also provided a research basis for further revealing the microbial mechanism involved in the soil ecological change process of rice-crayfish cocropping model.
维普
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