Effects of Microplastics on Morphological,Behavioral and Physiological Characteristics of Chinese Soft-Shelled Juvenile Turtle(Pelodiscus sinensis)
Microplastics are plastic fragments less than 5 mm in diameter that are difficult to degrade and potentially harmful to the ecosystem.At present,studies on the toxicological effects of microplastics on freshwater aquatic organisms mostly focus on zooplankton,fish,crustaceans and bivalves.In the present study,it explored the effects of microplastics on the embryonic stage of Chinese soft-shelled turtle(Pelodiscus sinensis)and different concentrations of microplastics on the morphology,behavior and physiological process of P.sinensis under laboratory conditions.Feeding different concentrations(0.1 mg/L,1 mg/L,10 mg/L)of microplastics did not significantly affect the morphological index(tergum and mass)and behavioral performance of the 14-days-ages,1-month-ages,3-month-ages and 6-month-ages juvenile P.sinensis.The results suggested that Proteobacteria,Bacteroidota,and Firmicutes the dominant bacteria in the gut microbiota of juvenile P.sinensis after ingested different concentrations of microplastics and gut bacterial community diversity did not change significantly.The abundance of gut microbiota of juvenile P.sinensis was significantly decreased when 0.1 mg/L microplastics were ingested.In addition,ingestion of different concentrations of microplastics could also change the enrichment of some opportunistic pathogens and some bacteria related to digestion,such as Bacteroides,Aeromonas,Cloacibacterium and so on.This research provides scientific and theoretical references for studying the toxic effects of microplastics on freshwater reptiles and assessing potential ecotoxicological risks of microplastics.
万方数据
维普
