Immunological Behavior of Peripheral Blood Mononuclear Cells on Mouse Blastocyst
The egg is fertilized a sperm forming a zygote,and then develops to form a blastocyst with series developmental events.Blastocyst hatches out of ZP and interacts with the uterus initiating implantation.It has been shown that embryo implantation is an inflammatory process.The genetic heterogeneity of the embryo determines that the embryo will trigger an immune response in the uterus.However,the immunological properties of the embryo have yet to be resolved.To investigate the possible immune interaction between embryos and immune cells,we use peripheral blood mononuclear cells(PBMC)to evaluate the immunological effects of mouse blastocysts.10 and 20 mouse blastocysts are co-cultured with PBMC for 24 hours,and lipopolysaccharide(LPS)treatment is used as a control respectively.Results show that embryos induce PBMC proliferation,in which the proportion of T cells is reduced significantly(P<0.05).When treated by LPS,NK cells and monocytes which are innate immune cells,PBMC are induced in proliferation.Treating both LPS and embryos,NK cells and monocytes are further proliferated(P<0.05).The cytokines in medium is analyzed by ELISA.Data show that the pro-inflammatory molecule IL-6 and the anti-inflammatory molecule IL-10 are significantly increased after PBMC are cultivated with embryos(P<0.05).Interestingly,embryos can down-regulate the expression of LPS-induced TNF-α(P<0.05).Results show that blastocysts have a regulatory effect on PBMC subpopulation,enhance LPS-triggered innate immune response,and regulate the secretion of cytokines IL-6,TNF-α and IL-10.Our findings imply that mouse blastocysts do not only cause an inflammatory response in the endometrium,but also have a dynamic regulatory effect on uterine immune cells.This study provides insights for further analysis of uterus-embryo immunological interaction during implantation.
国家科技期刊平台
NSTL
万方数据
