Effect of NO sustained-release nanoparticles PEG-b-PAASNO on pulmonary artery smooth muscle cells
Objective:To study the effect of nitric oxide(NO)donor nano-polymer micelle PEG-b-PAASNO(PS-NO)on pulmonary artery smooth muscle cells(PASMC).Methods:PSNO was constructed and its release rate was assayed in vitro.Rat PASMCs were isolated and cultured.The safe concentration range and effective concentration of PSNO were determined by CCK8.The cells were divided into control group,PSNO(250 ng·mL-1)group,plate-let-derivedgrowthfactor-BB(PDGF-BB,15 ng·mL-1)group(BB group),and PDGF-BB + PSNO(15 ng·mL-1 PDGF-BB,250 ng·mL-1 PSNO)group(BB + PSNO group).5-ethynyl-2'-deoxyuridine(EDU),Transwell and wound healing were used to examine the effects of PSNO on the proliferation and migration ability of PASMC.The expression levels of proliferation,contraction and cell cycle-related proteins[proliferating cell nuclear antigen(PC-NA),calponin,alpha-smooth muscle actin(α-SMA),cyclin D1and cyclin-dependent kinase 2(CDK2)]of PASMC were evaluated by Western blotting.Data were analyzed by one-way ANOVA analysis.Results:A nano-polymer micelle with stable NO release(PSNO)was constructed,and NO was continuously released for more than 48 h in a time-dose-dependent manner.When the concentration of PSNO was 250 ng·mL-1,proliferation and migration of PASMC induced by PDGF-BB were effectively inhibited.Compared with BB group,the expression levels of PCNA,Calponin,α-SMA,cyclin D1 and CDK2 in BB +PSNO group were significantly lower(P＜0.05).Conclusion:A nano-polymer micelle with stable release of NO(PSNO)is successfully constructed,which can effectively release NO for more than 48 h,blocking the proliferation and migration of PASMC,and reducing the expression level of PASMC contraction and cell cycle-related proteins.
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万方数据
维普
