高糖环境下XIST对人视网膜血管内皮细胞凋亡的影响
Effects of XIST on apoptosis of human retinal vascular endothelial cells under high glucose environment
张红振 1伏君 2戴琦 3岳肖 1艾合麦提麦麦提 1张婷 1米娜娃儿亚森1
作者信息
- 1. 新疆阿克苏地区第一人民医院 眼科,新疆 阿克苏 843000
- 2. 新疆医科大学第八附属医院 眼科,新疆 阿克苏 843000
- 3. 温州医科大学附属眼视光医院 眼科,浙江 温州 325027
- 折叠
摘要
目的:探讨高糖环境下长链非编码RNA X-非活性特异转录本(XIST)对人视网膜血管内皮细胞凋亡的影响及可能机制.方法:以人视网膜血管内皮细胞为研究对象,设对照组和高糖组,分析高糖对人视网膜血管内皮细胞XIST表达的影响;设对照组、高糖组、高糖+XIST组、高糖+阴性对照组,分析过表达XIST对高糖诱导人视网膜血管内皮细胞增殖和凋亡的影响;设对照组、高糖组、高糖+ XIST组、高糖+ XIST + pcD-NA3.1-Wnt1 组、高糖+XIST +pcDNA3.1-CON组,分析XIST通过Wnt1/β-catenin通路对高糖诱导人视网膜血管内皮细胞增殖和凋亡的影响.结果:(1)高糖组XIST相对表达量较对照组低(P＜0.05).(2)与对照组比,高糖组细胞增殖活力低,而凋亡率、Wnt1 和β-catenin水平高(P＜0.05);与高糖+阴性对照组比,高糖+XIST组增殖活力高,而凋亡率、Wnt1 和β-catenin水平低(P＜0.05).(3)与对照组比,高糖组细胞增殖活力低,而凋亡率高(P＜0.05);与高糖组比,高糖+XIST组增殖活力高,而凋亡率低(P＜0.05);与高糖+XIST + pcDNA3.1-CON组比,高糖+XIST +pcDNA3.1-Wnt1 组增殖活力低,而凋亡率高(P＜0.05).结论:XIST通过负调控Wnt1/β-catenin通路抑制高糖诱导的人视网膜血管内皮细胞凋亡.
Abstract
Objective:To investigate the effect and possible mechanism of long non-coding RNA X-inactive spe-cific transcript(XIST)on apoptosis of human retinal vascular endothelial cells under high glucose environment.Methods:Taking human retinal vascular endothelial cells as the research objects,firstly to analyze the effect of high glucose on the expression of XIST in human retinal vascular endothelial cells,the cells were divided into con-trol group and high glucose group.Then to analyze the effect of overexpression of XIST on the proliferation and ap-optosis of human retinal vascular endothelial cells induced by high glucose,the cells were divided into control group,high glucose group,high glucose +XIST group,and high glucosenegative control group.Finally in order to analyze the effect of XIST on the proliferation and apoptosis of human retinal vascular endothelial cells induced by high glucose through the Wnt1/β-catenin pathway,the cells were divided into control group,high glucose group,high glucose +XIST group,high glucose +XIST +pcDNA3.1-Wnt1 group,high glucose +XIST +pcDNA3.1-CON group.Results:(1)The relative expression of XIST in the high glucose group was lower than that in the control group(P＜0.05).(2)Compared with the control group,the cell proliferation activity in the high glucose group was lower,while the apoptosis rate,Wnt1 and β-catenin protein expression levels were higher(P＜0.05).The +XIST group had higher cell proliferation activity,while the apoptosis rate,Wnt1 and β-catenin protein expression levels were lower(P＜0.05).(3)Compared with the control group,the cell proliferation activity in the high glucose group was lower,but the apoptosis rate was higher(P＜0.05).Compared with the high glucose group,the cell proliferation activity in the high glucose +XIST group was higher,and the apoptosis rate was lower.Compared with the high glucose +XIST +pcDNA3.1-CON group,the high glucose +XIST +pcDNA3.1-Wnt1 group had lower cell proliferation activity and higher apoptosis rate(P＜0.05).Conclusion:XIST inhibits high glucose-induced apop-tosis of human retinal vascular endothelial cells by negatively regulating the Wnt1/β-catenin pathway.
关键词
长链非编码RNA/非活性特异转录本/人视网膜血管内皮细胞/凋亡Key words
lncRNA/inactive specific transcript/human retinal vascular endothelial cells/apoptosis引用本文复制引用
基金项目
省部共建中亚高发病成因与防治国家重点实验室资助项目(SKL-HIDCS-2023-AY6)
出版年
2024
NETL
NSTL
维普
万方数据