Effects of XIST on apoptosis of human retinal vascular endothelial cells under high glucose environment
Objective:To investigate the effect and possible mechanism of long non-coding RNA X-inactive spe-cific transcript(XIST)on apoptosis of human retinal vascular endothelial cells under high glucose environment.Methods:Taking human retinal vascular endothelial cells as the research objects,firstly to analyze the effect of high glucose on the expression of XIST in human retinal vascular endothelial cells,the cells were divided into con-trol group and high glucose group.Then to analyze the effect of overexpression of XIST on the proliferation and ap-optosis of human retinal vascular endothelial cells induced by high glucose,the cells were divided into control group,high glucose group,high glucose +XIST group,and high glucosenegative control group.Finally in order to analyze the effect of XIST on the proliferation and apoptosis of human retinal vascular endothelial cells induced by high glucose through the Wnt1/β-catenin pathway,the cells were divided into control group,high glucose group,high glucose +XIST group,high glucose +XIST +pcDNA3.1-Wnt1 group,high glucose +XIST +pcDNA3.1-CON group.Results:(1)The relative expression of XIST in the high glucose group was lower than that in the control group(P＜0.05).(2)Compared with the control group,the cell proliferation activity in the high glucose group was lower,while the apoptosis rate,Wnt1 and β-catenin protein expression levels were higher(P＜0.05).The +XIST group had higher cell proliferation activity,while the apoptosis rate,Wnt1 and β-catenin protein expression levels were lower(P＜0.05).(3)Compared with the control group,the cell proliferation activity in the high glucose group was lower,but the apoptosis rate was higher(P＜0.05).Compared with the high glucose group,the cell proliferation activity in the high glucose +XIST group was higher,and the apoptosis rate was lower.Compared with the high glucose +XIST +pcDNA3.1-CON group,the high glucose +XIST +pcDNA3.1-Wnt1 group had lower cell proliferation activity and higher apoptosis rate(P＜0.05).Conclusion:XIST inhibits high glucose-induced apop-tosis of human retinal vascular endothelial cells by negatively regulating the Wnt1/β-catenin pathway.
lncRNAinactive specific transcripthuman retinal vascular endothelial cellsapoptosis
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NSTL
万方数据
维普
