The impact of HMG CoA reductase ScrFI gene polymorphism on hypercholesterolemia and AI:a cross-sectional study of a middle-aged and elderly population
Objective:To explore the effect of 3-hydroxy-3-methyl glutaryl coenzyme A reductase(HMG CoA re-ductase)gene polymorphism on hypercholesterolemia and atherosclerosis index(AI).Methods:The total cholester-ol,high density lipoprotein cholesterol(HDL-C)and low density lipoprotein cholesterol(LDL-C)profiles in blood of 174 middle-aged and old people of Han nationality living in Nanjing were detected,and the AI was calculated.Pol-ymerase chain reaction-restriction endonuclease fragment length polymorphism(PCR-RFLP)technology was used to detect the polymorphism of HMG-CoA reductase ScrFI gene.Results:There were 87 patients with hypercholesterol-emia(high cholesterol group),including21 males and66 females,with an average age of(63.59±8.37)years;There were 87 cases with normal cholesterol level(normal group),including 21 males and 66 females,with an av-erage age of(62.92±7.60)years.In the high cholesterol group,the frequency of AA genotype was25.10%,the frequency of Aa genotype was 55.31%,and the frequency of aa genotype was 19.59%;In the normal group,the frequency of AA genotype was 18.39%,the frequency of Aa genotype was 52.87%,and the frequency of aa geno-type was 28.74%.The frequency of a allele in high cholesterol group was 55.17%,higher than 47.13%in normal group,the difference was not statistically significant(P>0.05).The level of blood HDL-C in AA genome popula-tion was significantly higher than that in Aa and aa genotype population(P<0.05).The AI of AA gene population in normal cholesterol group was significantly lower than that of aa gene population(P<0.05).The AI of the two groups gradually increased with the occurrence of mutant heterozygotes and mutant homozygotes,the difference was not statistically significant(P>0.05).Conclusion:The polymorphism of HMG-CoA reductase ScrFI gene may be one of the genetic predisposing factors of hypercholesterolemia.HMG-CoA reductase ScrFI gene mutation may affect the activity of HMG-CoA reductase,reduce the inhibition of cholesterol,and increase the synthesis of TC and LDL-C,exposed to the same environment as the non-mutant population,the risk of hypercholesterolemia and atheroscle-rosis may be increased.
3-hydroxy-3-methyl glutaryl coenzyme A reductaseScrFI genotypeatherosclerosis indexhyper-cholesterolemiamiddle-aged and elderly people
NETL
NSTL
万方数据
维普
