非小细胞肺癌组织LncRNA CRNDE表达及临床意义
Expression and clinical significance of LncRNA CRNDE in non-small cell lung cancer
张效亮 1刘晓娇 1马小婷 1张超1
作者信息
- 1. 中国人民解放军联勤保障部队第九四○医院 放射治疗科,甘肃 兰州 730050
- 折叠
摘要
目的:分析长链非编码RNA结直肠肿瘤差异表达基因(LncRNA CRNDE)在非小细胞肺癌(NSCLC)组织中的表达及临床病理意义.方法:2020 年2 月至 2023 年 5 月,来自我院的 63 例NSCLC患者被纳入研究.采用实时定量聚合酶链反应(RT-qPCR)检测肿瘤组织和邻近正常组织中LncRNA CRNDE的表达.利用本研究队列和TCGA队列分析LncRNA CRNDE的临床病理意义和预后价值.结果:RT-qPCR分析显示,LncRNA CRNDE在肿瘤组织中的表达显著高于邻近的正常组织[3.04(0.56,13.04)vs.0.24(0.05,1.99),P<0.001].在受试者工作特征曲线中,LncRNA CRNDE是有能力区分NSCLC肿瘤组织和邻近正常组织的,曲线下面积为0.713(P<0.001),特异度较高,为81.0%.肿瘤组织LncRNA CRNDE在TNM Ⅲ期中表达水平高于Ⅰ～Ⅱ期患者(P =0.001).TCGA数据显示,与邻近的正常组织(n =106)相比,LncRNA CRNDE在NSCLC肿瘤组织(n =1 014)中呈高表达[41.00(24.00,72.00)vs.22.60(10.60,47.70),P<0.001].Ln-cRNA CRNDE高表达与所有患者较短的总生存(OS)时间以及肺鳞癌患者较短的OS时间和首次进展时间有关(P<0.05).多变量生存分析结果也表明,N2/3分期(P =0.004)和LncRNA CRNDE高表达(P =0.010)与NSCLC患者较短的OS时间显著相关.结论:LncRNA CRNDE有希望作为NSCLC的潜在诊断标志物和治疗靶点.
Abstract
Objective:To analyze the expression and clinical pathological significance of long non-coding RNA colorectal neoplasia differentially expressed(LncRNA CRNDE)in non-small cell lung cancer(NSCLC)tissues.Methods:From February 2020 to May 2023,63 NSCLC patients from our hospital were included in the study.Re-al time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of LncRNA CRNDE in tumor tissues and adjacent normal tissues.Then,the clinical pathological significance and prognostic value of Ln-cRNA CRNDE were analyzed using this study cohort and TCGA cohort.Results:Through RT-qPCR analysis,the expression of LncRNA CRNDE in tumor tissues was significantly higher than that in adjacent normal tissues[3.04(0.56,13.04)vs.0.24(0.05,1.99),P<0.001].In the receiver operating characteristic curve of the sub-jects,LncRNA CRNDE had the ability to distinguish between NSCLC tumor tissue and adjacent normal tissue,with an area under the curve of 0.713(P<0.001)and a high specificity of 81.0%.The expression level of LncRNA and CRNDE in tumor tissue was higher in TNM stage Ⅲ than that in patients with stageⅠ-Ⅱ(P =0.001).Simi-larly,by analyzing TCGA data,LncRNA CRNDE was highly expressed in NSCLC tumor tissue(n =1 014)com-pared to adjacent normal tissues(n =106)[41.00(24.00,72.00)vs.22.60(10.60,47.70),P<0.001].The high expression of LncRNA CRNDE was associated with a shorter overall survival(OS)time for all patients,as well as a shorter OS time and first progression time for lung squamous cell carcinoma patients(P<0.05).The results of multivariate survival analysis also showed that N2/3 staging(P =0.004)and high expression of LncRNA CRNDE(P =0.010)were significantly correlated with shorter OS time in NSCLC patients.Conclusion:LncRNA CRNDE has potential as a diagnostic marker and therapeutic target for NSCLC.
关键词
非小细胞肺癌/长链非编码RNA/结直肠肿瘤差异表达基因/TCGA/预后Key words
non-small cell lung cancer/long non-coding RNA/colorectal neoplasia differentially expressed/TC-GA/prognosis引用本文复制引用
基金项目
甘肃省科技计划项目(22JR5RA003)
出版年
2024
NETL
NSTL
维普
万方数据