Expression of GLAST and GS protein in HBV associated hepatocellular carcinoma tissue and their relationship with early postoperative recurrence and metastasis
Objective:To detect the expression of excitatory amino acid transporter 1(GLAST)and glutamine synthetase(GS)protein in hepatitis B virus(HBV)associated hepatocellular carcinoma(HCC)and analyze on their relationships with early recurrence and metastasis after resection.Methods:125 patients with HBV associated HCC underwent resection surgery in our hospital from March 2020 to May 2022 were selected.After surgery,the expres-sion of GLAST and GS protein in cancer tissue and normal tissue at the cutting edge was detected using immunohis-tochemistry.The positive expression rates of GLAST and GS protein between cancer tissue and normal tissue at the cutting edge were compared.The positive expression rates of GLAST and GS proteins in cancer tissues of patients with different clinical and pathological characteristics were compared.After following-up for 1 year,Cox regression analysis of influencing factors for recurrence and metastasis were conducted.Results:The positive expression rates of GLAST and GS protein in cancer tissue were 28.57%and 73.95%,respectively.The former was lower than 57.98%of normal tissue at the cutting edge,while the latter was higher than 33.61%of normal tissue at the cut-ting edge(P<0.05).The positive expression rate of GLAST protein in cancer tissue of patients with HBV infection over 22 years was lower than those of patients with HBV infection less than 10 years,10-22 years(P<0.05).The positive expression rates of GLAST protein in cancer tissues of tumor node metastasis(TNM)Ⅲ-Ⅳ stage,patients with portal vein tumor thrombus,and undifferentiated/poorly differentiated patients were lower than those of TNM Ⅰ-Ⅱ stage,patients without portal vein tumor thrombus,and patients with medium/high differentiated(P<0.05),and the trend of GS protein positive rates was opposite.The incidence of early postoperative recurrence and metastasis was 21.01%.Age(HR =1.471,95%CI 1.086-1.993),accompanied by cirrhosis(HR =1.728,95%CI 1.110-2.691),positive expression of GLAST protein(HR =0.451,95%CI 0.224-0.910),and posi-tive expression of GS protein(HR =2.255,95%CI 1.027-4.948)were all influencing factors for early postopera-tive recurrence and metastasis in patients(P<0.05).Conclusion:The positive expression rate of GLAST protein in HBV associated HCC tissue is low,while the positive expression rate of GS protein is high,and both are related to early recurrence and metastasis after resection.
hepatitis B virushepatocellular carcinomaexcitatory amino acid transporter 1glutamine syn-thetaserecurrence and metastasis
NETL
NSTL
万方数据
维普
