Prognostic significance of the ratio of cytoplasmic-cytonuclear AR expression in patients with cervical adenocarcinoma
Objective:To investigate the expression pattern of androgen receptor(AR)in cervical adenocarcinoma and the relationship between AR expression and prognosis using immunohistochemistry.Methods:Immunohisto-chemical staining for AR was performed on normal cervical tissue specimens from 30 patients who underwent total hysterectomy for benign diseases such as adenomyosis and 86 tumor tissue specimens from patients who underwent total hysterectomy for cervical adenocarcinoma.General information of the subjects was collected.The relationship between each clinicopathological feature and patient survival was assessed on the basis of AR expression.Results:83％(25/30)of normal cervical tissues showed positive nuclear staining,whereas compared to normal cervical tis-sues,cervical adenocarcinoma tumor tissues showed different modes of expression of AR,with 94％(81/86)of pa-tients AR positive,of which 82％(66/81)showed nuclear-plasmic double staining,12％(10/81)showed a clear single cytoplasmic positivity,and only 6％(5/81)showed AR nuclear staining similar to normal cervical tissue.In patients with nuclear-plasmic double staining,cytoplasmic staining was more noticeable in AR.We measured the expression of AR utilizing the cytoplasmic-cytonuclear ratio in order to more precisely characterize its expression in various subcellular localizations of tumor cells.The ratio＞2.5 group had more patients with positive lymph nodes(P＜0.01)and deep myometrial invasion(P＜0.01)compared to the ratio≤2.5 group.Additionally,the ratio＞2.5 group had a higher incidence of vimentin positivity(P＜0.01).Kaplan-Meier analyses revealed a higher cyto-plasmic-to-cytonuclear ratio of AR expression associated with lower survival rates.Univariate and multivariate Cox regression analyses identified it as an independent prognostic factor for distant metastasis.Conclusion:The cyto-plasmic-to-cytonuclear ratio of AR expression is an independent prognostic factor,offering potential as a predictor for the risk of distant metastasis in cervical adenocarcinoma patients.
万方数据
维普
