Diagnostic value of ultrasound combined with serum miR-106a-5p,miR-20b-5p for lower extremity vascular disease of diabetic foot
Objective:To explore the diagnostic value of ultrasound combined with serum miR-106a-5p,miR-20b-5p for lower extremity vascular disease(LEVD)of diabetic foot(DF).Methods:Ninety patients with DF who visited our hospital from March 2021 to June 2023 were selected as the study subjects.They were grouped into a LEVD group of 42 cases and a non LEVD group of 48 cases based on whether they were complicated with LEVD.Real-time fluorescence quantitative PCR was applied to detect the expression levels of miR-106a-5p and miR-20b-5p in serum of DF patients,and lower extremity vascular ultrasound examination was performed on all patients.The diagnostic value of serum miR-106a-5p and miR-20b-5p in DF patients with LEVD was analyzed using the receiver operating characteristic(ROC)curve;the consistency Kappa test was applied to analyze the consistency between ul-trasound alone and in combination of serum miR-106a-5p,miR-20b-5p,with the gold standard.Results:The con-sistency between the ultrasound examination results and the gold standard diagnostic results was high,with a Kappa value of 0.642(P＜0.05).The serum levels of miR-106a-5p and miR-20b-5p in the LEVD group were obviously higher than those in the non LEVD group(P＜0.05).The area under the curve(AUC)of serum miR-106a-5p and miR-20b-5p for diagnosing LEVD in DF patients was 0.803 and 0.757,respectively.The consistency between the serum miR-106a-5p,miR-20b-5p test results and the gold standard diagnostic results was moderate,with Kappa values of 0.503 and 0.492,respectively(P＜0.05).The results of ultrasound combined with serum miR-106a-5p,miR-20b-5p levels were highly consistent with the gold standard diagnostic results,with a Kappa value of 0.866(P＜0.05).The accuracy,and negative predictive value of ultrasound combined with serum miR-106a-5p and miR-20b-5p in diagnosing LEVD in DF patients were obviously higher than those of the three alone(P＜0.05).Conclusion:Ultrasound combined with serum miR-106a-5p and miR-20b-5p have high diagnostic value for LEVD in DF patients and are worth promoting.
diabetic footlower extremity vascular diseaseultrasoundmicroRNA-106a-5pmicroRNA-20b-5pdiagnostic value
万方数据
维普
