Expression of ALKBH5 in hepatocellular carcinoma and its effect on anti-tumor immunity
Objective:To investigate the expression of ALKBH5 and its effect on antitumor immunity in liver cancer.Methods:The expression of ALKBH5 in 50 patients of HCC and corresponding adjacent nontumor tissues was detected by immunohistochemical staining(IHC),and the correlations between ALKBH5 and clinical features were analyzed byx2 test.The relationships between ALKBH5 and the survival of HCC patients was evaluated by Ka-plan-Meier method.GSEA,GO,KEGG,and Reactome pathway enrichment analysis were applied to predict the potential mechanism of ALKBH5 involved.The relationship between ALKBH5 mRNA and immune cell infiltration and immune checkpoint molecules was analyzed in TIMER and TISIDB databases.Results:ALKBH5 was downreg-ulated in HCC tissue,and the expression level of ALKBH5 were lower in HCC tissues with tumor size＞5 cm,or with infiltration of tumor encapsulation,or ES grade Ⅲ/Ⅳ.Patients with lower ALKBH5 showed poor overall sur-vival(OS)and progression free survival(PFS).Moreover,gene enrichment analysis showed that ALKBH5 might participate in immune related pathways.ALKBH5 was correlated with the infiltration of immune cells,such as CD4+T,CD8+T,DC,NK,and the expression of PD-1/L1 and CTLA4.Conclusion:ALKBH5 can promote ma-lignant progression of HCC by affecting its antitumor immunity,resulting in patient poor prognosis.
hepatocellular carcinomam6 A methylationALKBH5anti-tumor immunity
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