目的:分析热休克蛋白家族 H(Hsp110)成员 1[heat shock protein family H(Hsp110)member 1,HSPH1]在宫颈癌组织中的表达及其与预后的相关性.方法:收集2018年2月至2021年1月首次确诊的60例宫颈癌患者的癌组织和相邻非癌组织.通过qRT-PCR检测HSPH1表达,分析其与肿瘤标志物、宫颈癌病理特征之间的关系,并通过Cox回归模型确定3年生存的独立预后因子.利用时间依赖性受试者工作特征(ROC)曲线评估HSPH1预测1年、2年和3年生存的临床价值,并通过外部数据库和细胞实验验证结果.结果:HSPH1在临床样本和GSE29570数据库宫颈癌组织中均呈高表达(P＜0.05),且与CA125、CA19-9、CEA和SCCA呈正相关(均r＞0,P＜0.05).高表达组(≥1.57)肿瘤大小≥4 cm、FIGOⅢ～Ⅳ期、淋巴结转移及淋巴血管空间侵犯比例均高于低表达组(P＜0.05).TCGA数据库和临床样本分析显示,高表达组患者生存率显著低于低表达组(P＜0.01).多因素Cox回归分析显示,HSPH1＜1.57(HR=0.299,P=0.039)和无淋巴结转移(HR=0.245,P=0.003)是独立预后因子.HSPH1预测1年、2年和3年生存的曲线下面积分别为0.82、0.85和0.88.结论:HSPH1在宫颈癌组织中的高表达与患者较差的预后之间存在显著相关,HSPH1可作为宫颈癌预后评估和治疗靶点的潜在生物标志物.
Expression of heat shock-associated protein HSPH1 in patients with cervical cancer and its correlation analysis with prognosis
Objective:To analyze the expression of heat shock protein family H(Hsp1 10)member 1(HSPH1)in cervical cancer tissues and its correlation analysis with prognosis.Methods:Cancerous tissues and adjacent non-cancerous tissues of 60 cervical cancer patients diagnosed for the first time from February 2018 to January 2021 were collected.HSPH1 expression was detected by qRT-PCR,its association with tumor markers was assessed,the asso-ciation between HSPH1 and pathological features of cervical cancer was analyzed,and independent prognostic fac-tors for three-year survival were determined by Cox regression model.The clinical value of HSPH1 in predicting 1-,2-and 3-year survival was assessed using time-dependent ROC curves,and the results were validated by external databases and cellular experiments.Results:HSPH1 was highly expressed in cervical cancer tissues in clinical samples and the GSE29570 database(P＜0.05)and positively correlated with CA125,CA19-9,CEA and SCCA(all r＞0,P＜0.05).The proportions of tumor≥4 cm,FIGO stage Ⅲ-Ⅳ,lymph node metastasis and lymphovas-cular space invasion were higher in the high-expression group(≥ 1.57)than in the low-expression group(P＜0.05).Analysis of the TCGA database and clinical samples showed that the survival rate of patients in the high-ex-pression group was significantly lower than that in the low-expression group(P＜0.01).Multifactorial Cox regres-sion analysis showed that HSPH1＜1.57(HR=0.299,P=0.039)and without lymph node metastasis(HR=0.245,P=0.003,)were independent prognostic factors.The area under the curve for HSPH1 predicting 1-,2-,and 3-year survival was 0.82,0.85,and 0.88,respectively.Conclusion:High expression of HSPH1 in cervical cancer tissues is significantly associated with the patients'poorer prognosis,suggesting that HSPH1 may serve as a potential biomarker for prognostic assessment and therapeutic targeting in cervical cancer.
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