薰衣草DXR基因在转基因烟草中的功能分析
Functional Analysis of Lavandula angustifolia DXR Gene in Transgenic Tobacco
王振昱 1苏秀娟2
作者信息
- 1. 新疆农业大学农学院,新疆维吾尔自治区 乌鲁木齐 830000
- 2. 新疆作物遗传改良与种质创新重点实验室,新疆维吾尔自治区 乌鲁木齐 830000
- 折叠
摘要
揭示薰衣草DXR基因的生物学功能,将为薰衣草精油性状改良提供科学依据.基于此,以烟草为例,利用农杆菌介导法对烟草进行了DXR基因的遗传转化,获得了10株转DXR基因的烟草植株,通过荧光定量技术确定了其在不同转基因植株中的表达量,利用GC-MS技术测定了转基因植株叶片中萜类挥发物的含量情况,为解析薰衣草DXR基因在萜类化合物代谢中的作用提供了依据.
Abstract
Revealing the biological function of DXR gene in Lavandula angustifolia will provide scientific basis for improving the character of lavender essential oil.Based on this,taking tobacco as an example,Agrobacterium-mediated DXR gene genetic transfor-mation was carried out on tobacco,and 10 tobacco plants with DXR gene transfer were obtained.Their expression levels in different transgenic plants were determined by fluorescence quantitative technique,and the contents of terpenoid volatile substances in the leaves of transgenic plants were measured by GC-MS technique.This study provided a basis for elucidating the role of DXR gene in the metabolism of terpenoid compounds in Lavandula angustifolia.
关键词
薰衣草/DXR基因/萜类化合物/功能验证Key words
Lavandula angustifolia/DXR gene/terpenoid/functional verification引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据