不同激素配比对小草海桐组培技术的影响研究
Study on the Influence of Different Hormone Ratio on Tissue Culture Technology of Scaevola hainanensis
高伟勤 1王佳 1童升洪 1赵秀芳1
作者信息
- 1. 岭南生态文旅股份有限公司,广东 东莞 523125
- 折叠
摘要
以半红树植物小草海桐叶片为外植体,MS为基础培养基,设计不同质量浓度浓度的6-BA、NAA和6-BA等激素,探究不同阶段小草海桐组培技术的最适宜浓度.结果表明,愈伤组织诱导阶段MS+0.5 mg/L NAA+0.1 mg/L 6-BA的培养基效果最好,诱导率为82.01%;在不定芽的诱导阶段,MS+1.0 mg/L 6-BA的培养基效果最好,出芽率为60.42%;在生根培养过程中,MS+0.5 mg/L IAA的培养基效果最好,生根率为87.67%.
Abstract
Using the leaves of the semi-mangrove plant Scaevola hainanensis as explants and MS as the base medium,different con-centrations of hormones such as 6-BA,NAA and 6-BA were designed to explore the optimal concentration of tissue culture technolo-gy of Scaevola hainanensis in different stages.The results showed that MS+0.5 mg/L NAA+0.1 mg/L6-BA medium had the best ef-fect on callus induction stage,and the induction rate was 82.01%.In the stage of bud induction,MS+1.0 mg/L6-BA medium had the best effect,and the budding rate was 60.42%.In the process of rooting culture,MS+0.5 mg/L IAA medium had the best effect,and the rooting rate was 87.67%.
关键词
半红树/小草海桐/组织培养/激素Key words
semimangrove plant/Scaevola hainanensis/tissue culture/hormone引用本文复制引用
基金项目
东莞市社会发展科技项目(20221800905472)
岭南股份科技创新项目(KY-0093)
出版年
2024
NETL
NSTL
万方数据