Study on the Influence of Different Hormone Ratio on Tissue Culture Technology of Scaevola hainanensis
Using the leaves of the semi-mangrove plant Scaevola hainanensis as explants and MS as the base medium,different con-centrations of hormones such as 6-BA,NAA and 6-BA were designed to explore the optimal concentration of tissue culture technolo-gy of Scaevola hainanensis in different stages.The results showed that MS+0.5 mg/L NAA+0.1 mg/L6-BA medium had the best ef-fect on callus induction stage,and the induction rate was 82.01%.In the stage of bud induction,MS+1.0 mg/L6-BA medium had the best effect,and the budding rate was 60.42%.In the process of rooting culture,MS+0.5 mg/L IAA medium had the best effect,and the rooting rate was 87.67%.