首页|不同激素配比对小草海桐组培技术的影响研究

不同激素配比对小草海桐组培技术的影响研究

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以半红树植物小草海桐叶片为外植体,MS为基础培养基,设计不同质量浓度浓度的6-BA、NAA和6-BA等激素,探究不同阶段小草海桐组培技术的最适宜浓度.结果表明,愈伤组织诱导阶段MS+0.5 mg/L NAA+0.1 mg/L 6-BA的培养基效果最好,诱导率为82.01%;在不定芽的诱导阶段,MS+1.0 mg/L 6-BA的培养基效果最好,出芽率为60.42%;在生根培养过程中,MS+0.5 mg/L IAA的培养基效果最好,生根率为87.67%.
Study on the Influence of Different Hormone Ratio on Tissue Culture Technology of Scaevola hainanensis
Using the leaves of the semi-mangrove plant Scaevola hainanensis as explants and MS as the base medium,different con-centrations of hormones such as 6-BA,NAA and 6-BA were designed to explore the optimal concentration of tissue culture technolo-gy of Scaevola hainanensis in different stages.The results showed that MS+0.5 mg/L NAA+0.1 mg/L6-BA medium had the best ef-fect on callus induction stage,and the induction rate was 82.01%.In the stage of bud induction,MS+1.0 mg/L6-BA medium had the best effect,and the budding rate was 60.42%.In the process of rooting culture,MS+0.5 mg/L IAA medium had the best effect,and the rooting rate was 87.67%.

semimangrove plantScaevola hainanensistissue culturehormone

高伟勤、王佳、童升洪、赵秀芳

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岭南生态文旅股份有限公司,广东 东莞 523125

半红树 小草海桐 组织培养 激素

东莞市社会发展科技项目岭南股份科技创新项目

20221800905472KY-0093

2024

农业技术与装备
山西省农业机械化技术推广总站 山西省农业技术推广站 山西省农业生态环境建设总站

农业技术与装备

影响因子:0.132
ISSN:1673-887X
年,卷(期):2024.(3)
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