Representative diseased leaves were collected from greenhouse,and the pathogens were isolated from the diseased plants by tissue separation method.The pure strains were isolated and purified on PDA,and the pathogenic bacteria were identified by pathogenicity test.DNA was extracted using kits and amplified by PCR using primers(ITS1 and ITS4).After obtaining the sequenc-es,the sequences were copied to the NCBI official website for Blast analysis,homology comparison was performed in GenBank,and the strains with close homology were downloaded.Phylogenetic tree was constructed by MAGE 7.0.After identification,it was found that the pathogen of two kinds of anthracnose was Fusarium equiseti,to provide data support for the prevention and control of strawberry diseases.
Xanthomonas fragariceFusarium equisetiisolation of pathogenic bacteria
维普
万方数据
