农业技术与装备2024，Issue(7) ：201-204.DOI:10.3969/j.issn.1673-887X.2024.07.075

草莓叶斑病真菌性病原菌的分离与鉴定

Isolation and Identification of Fungal Pathogens from Xanthomonas fragarice Strawberry Leaves

王月霞 石爱 韩世明 杨寒丽
农业技术与装备2024，Issue(7) ：201-204.DOI:10.3969/j.issn.1673-887X.2024.07.075
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  • 万方数据

草莓叶斑病真菌性病原菌的分离与鉴定

Isolation and Identification of Fungal Pathogens from Xanthomonas fragarice Strawberry Leaves

王月霞 1石爱 1韩世明 1杨寒丽2
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作者信息

  • 1. 六盘水师范学院生物科学与技术学院,贵州 六盘水 553004
  • 2. 巴音郭楞蒙古自治州农业技术推广中心,新疆维吾尔自治区 库尔勒 841000
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摘要

从新疆和静县巴润哈尔莫墩镇草莓棚采集具有代表性的病叶,采用组织分离法从病株将病菌分离出来,在PDA上分离纯化获得纯种菌株,先利用致病性实验鉴定病原菌,再利用试剂盒提取DNA,通过引物(ITS1和ITS4)进行PCR扩增.得到序列后,将序列复制到NCBI官网中,进行Blast分析,在GenBank中进行同源性比对,下载同源性比较近的菌株,再利用MAGE 7.0构建系统发育树.经过鉴定得知,采样所得的2种叶斑病的病原菌是木贼镰刀菌,为防治草莓病害提供数据支持.

Abstract

Representative diseased leaves were collected from greenhouse,and the pathogens were isolated from the diseased plants by tissue separation method.The pure strains were isolated and purified on PDA,and the pathogenic bacteria were identified by pathogenicity test.DNA was extracted using kits and amplified by PCR using primers(ITS1 and ITS4).After obtaining the sequenc-es,the sequences were copied to the NCBI official website for Blast analysis,homology comparison was performed in GenBank,and the strains with close homology were downloaded.Phylogenetic tree was constructed by MAGE 7.0.After identification,it was found that the pathogen of two kinds of anthracnose was Fusarium equiseti,to provide data support for the prevention and control of strawberry diseases.

关键词

草莓叶斑病/木贼镰刀菌/病原菌分离

Key words

Xanthomonas fragarice/Fusarium equiseti/isolation of pathogenic bacteria

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基金项目

六盘水师范学院项目(LPSSYKYJJ201601)

六盘水师范学院项目(LPSSY2023XKTD09)

六盘水市科技局项目(52020-2020-0906)

出版年

2024
农业技术与装备
山西省农业机械化技术推广总站 山西省农业技术推广站 山西省农业生态环境建设总站

农业技术与装备

影响因子：0.132
ISSN：1673-887X
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