In order to investigate the effect of organic fertilizer replacing chemical fertilizer on the yield of winter wheat,this paper used different proportions of bio-organic fertilizer and chemical fertilizer to fertilize winter wheat,and compared the growth period,main characters and yield of different treatments of wheat.The results showed that the combined application of bio-organic fertilizer and inorganic fertilizer had no significant effect on the whole growth period of winter wheat.With the increase of the application amount of bio-organic fertilizer,the wheat yield was first increased and then decreased,and the wheat yield of treatment 5(156 kg/hm2 organic fertilizer nitrogen and 39 kg/hm2 inorganic fertilizer nitrogen)was the highest.